- SYNTHESIS OF MULTI-13C-ENRICHED METHOTREXATE FOR NMR STUDIES OF DRUG - ENZYME INTERACTIONS
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Methotrexate N--L-glutamic acid, 13C-enriched at positions 6; 2,7,9; 4,7,8a,9; and 2,4a,6 were synthesized from the correspondingly labelled 2,4-diamino-6-methylpteridine by "benzylic" bromination followed by displacement of the bromide by di-t-butyl N-(p-methylaminobenzoyl)-L-glutamate.Acid treatment of each methotrexate di-t-butyl ester formed yielded the corresponding 13C-enriched methotrexate.
- Cheung, H.T. Andrew,Smal, Mary,Chau, Diem Dieu
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p. 507 - 514
(2007/10/02)
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