Oriented immobilization of farnesylated proteins by the thiol-ene reaction
figure presented Anchoring the protein: Proteins were immobilized rapidly under mild conditions by thiol-ene photocoupling between S-farnesyl groups attached to a genetically encodable CAAX-box tetrapeptide sequence (A is aliphatic) at the C terminus of the protein and surface-exposed thiols (see scheme). This method enables the oriented covalent immobilization of proteins directly from expression lysates without additional purification or derivatization steps.
Weinrich, Dirk,Lin, Po-Chiao,Jonkheijm, Pascal,Nguyen, Uyen T. T.,Schroeder, Hendrik,Niemeyer, Christof M.,Alexandrov, Kirill,Goody, Roger,Waldmann, Herbert