Spectroscopic studies on the inclusion behavior between caffenic acid and γ-cyclodextrin
To investigate the inclusion ability of γ-cyclodextrin (γ-CD) for caffenic acid (CA). The conditions for the formation of inclusion complex and the binding constant between γ-CD and CA were determined by fluorescent and ultraviolet spectroscopic methods. The behavior of CA as a free radical scavenger before and after its inclusion was investigated. In addition, solid samples of the inclusion complex, prepared through the co-precipitation and grinding methods, were characterized via IR spectroscopy and differential scanning calorimetry. The inclusion complex was further characterized with 1H NMR spectroscopy. By using fluorescent and ultraviolet spectroscopy, the conditions for the formation of inclusion complex between γ-CD and CA were optimized and the binding constant determined. It was observed that the guest molecule behaves as a better anti-oxidant after its inclusion into γ-CD.