- Efficient isolation and accurate in situ analysis of circulating tumor cells using detachable beads and a high-pore-density filter
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Analysis of cancer cells: A new technique isolates rare circulating tumor cells (CTCs) and analyzes their protein expression by the use of detachable beads and high-pore-density filters. This technique shows significantly improved efficiency in the isolat
- Lee, Hun Joo,Oh, Jin Ho,Oh, Jin Mi,Park, Jong-Myeon,Lee, Jeong-Gun,Kim, Minseok S.,Kim, Yeon Jeong,Kang, Hyun Ju,Jeong, Joon,Kim, Seung Il,Lee, Soo Suk,Choi, Jeong-Woo,Huh, Nam
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Read Online
- MASSIVE PARALLEL METHOD FOR DECODING DNA AND RNA
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This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
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Paragraph 0042; 0150
(2016/12/16)
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- Cleavable biotin probes for labeling of biomolecules via azide-alkyne cycloaddition
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The azide-alkyne cycloaddition provides a powerful tool for bio-orthogonal labeling of proteins, nucleic acids, glycans, and lipids. In some labeling experiments, e.g., in proteomic studies involving affinity purification and mass spectrometry, it is convenient to use cleavable probes that allow release of labeled biomolecules under mild conditions. Five cleavable biotin probes are described for use in labeling of proteins and other biomolecules via azide-alkyne cycloaddition. Subsequent to conjugation with metabolically labeled protein, these probes are subject to cleavage with either 50 mM Na 2S2O4, 2% HOCH2CH2SH, 10% HCO2H, 95% CF3CO2H, or irradiation at 365 nm. Most strikingly, a probe constructed around a dialkoxydiphenylsilane (DADPS) linker was found to be cleaved efficiently when treated with 10% HCO 2H for 0.5 h. A model green fluorescent protein was used to demonstrate that the DADPS probe undergoes highly selective conjugation and leaves a small (143 Da) mass tag on the labeled protein after cleavage. These features make the DADPS probe especially attractive for use in biomolecular labeling and proteomic studies.
- Szychowski, Janek,Mahdavi, Alborz,Hodas, Jennifer J. L.,Bagert, John D.,Ngo, John T.,Landgraf, Peter,Dieterich, Daniela C.,Schuman, Erin M.,Tirrell, David A.
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experimental part
p. 18351 - 18360
(2011/03/18)
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