- An in-situ detection of biological in vivo adjacent mercat protein compound and its method of
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The invention belongs to the technical field of biological detection, and specifically relates to a compound and a method for in-situ detecting o-sulfhydryl protein in an organism. A kind of micromolecular fluorescent probes capable of specifically detecting o-sulfhydryl of protein are designed and synthesized, and a method for in-situ fluorescence labeling and detection of endogenous o-sulfhydryl protein in the organism is built by combining fluorescence polarization technology, protein fluorescent electrophoresis and confocal fluorescence microtechnique.
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Paragraph 0087; 0088
(2016/12/01)
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- Versatile probes for the selective detection of vicinal-dithiol-containing proteins: Design, synthesis, and application in living cells
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Endogenous vicinal-dithiol-containing proteins (VDPs) that have two thiol groups close to each other in space play a significant importance in maintaining the cellular redox microenvironment. Approaches to identify VDPs mainly rely on monitoring the different concentration of monothiol and total thiol groups or on indirect labeling of vicinal thiols by using p-aminophenylarsenoxide (PAO). Our previous work has reported the direct labeling of VDPs with a highly selective receptor PAO analogue, which could realize fluorescence detection of VDPs directly in living cells. Herein, we developed a conjugated approach to expand detectable tags to nitrobenzoxadiazole (NBD), fluorescein, naphthalimide, and biotin for the synthesis of a series of probes. Different linkers have also been introduced toward conjugation of VTA2 with these functional tags. These synthesized flexible probes with various features will offer new tools for the potential identification and visualization of vicinal dithiols existing in different regions of VDPs in living cells. These probes are convenient tools for proteomics studies of various disease-related VDPs and for the discovery of new drug targets. Copyright
- Huang, Chusen,Yin, Qin,Meng, Jiangjiang,Zhu, Weiping,Yang, Yi,Qian, Xuhong,Xu, Yufang
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supporting information
p. 7739 - 7747
(2013/07/19)
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- A simple FRET-based modular design for diagnostic probes
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In recent years, there has been a massive effort to develop molecular probes with optical modes of action. Probes generally produce detectable signals based on changes in fluorescence properties. Here, we demonstrate the potential of self-immolative molecular adaptors as a platform for Turn-On probes based on the FRET technique. The probe is equipped with identical fluorophore pairs or a fluorophore/quencher FRET pair and a triggering substrate. Upon reaction of the analyte of interest with the triggering substrate, the self-immolative adaptor spontaneously releases the two dye molecules to break off the FRET effect. As a result, a new measurable fluorescent signal is generated. The fluorescence obtained can be used to quantify the analyte. The modular structure of the probe design will allow the preparation of various chemical probes based on the FRET activation technique.
- Redy, Orit,Kisin-Finfer, Einat,Sella, Eran,Shabat, Doron
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supporting information; scheme or table
p. 710 - 715
(2012/02/05)
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- Xanthamide fluorescent dyes
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Two derivatives of fluorescein, termed "xanthamides," were prepared from fluorescein, an inexpensive dye. Relative to fluorescein, which contains a 6-phenolic OH and a 2′-carboxyl, the first derivative (5) contains a carboxymethyl ether at the 6-position
- Gao, Jianxin,Wang, Poguang,Giese, Roger W.
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p. 6397 - 6401
(2007/10/03)
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