An efficient enzymatic synthesis of thiamin pyrophosphate
Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.
Melnick, Jonathan S.,Sprinz, K. Ingrid,Reddick, Jason J.,Kinsland, Cynthia,Begley, Tadhg P.
Preparation method of co-carboxylase and tetrahydrate or salt thereof
The invention discloses a preparation method of co-carboxylase and tetrahydrate or salt thereof. The invention provides a preparation method of a co-carboxylase shown as a formula I. The method comprises the following steps: eluting a mixture containing the co-carboxylase as shown in the formula I by using weakly-alkaline anion exchange resin through water and a weakly-alkaline aqueous solution with the pH value of 10-12 as eluent in sequence, and collecting eluate to obtain an aqueous solution containing the co-carboxylase as shown in the formula I, wherein the mixture containing the co-carboxylase as shown in the formula I comprises thiamine monophosphate and the co-carboxylase as shown in the formula I.