- Sulfo - Cy3 carboxylic acid fluorescent dye and preparation process thereof
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The invention relates to a sulfo - Cy3 carboxylic acid fluorescent dye and a preparation process thereof, wherein the synthesized sulfo - Cy3 carboxylic acid has fluorescent quantum yield. At the same time, the high-emission high-excitation wavelength and is easily dissolved in water physical and chemical property stability points, and simultaneously, a single condensation target product needs to be selectively generated when the indole α-position methyl activity is achieved through simultaneous construction of the benzylindole two-side branch chains and high in yield, and C18 column separation is avoided.
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Paragraph 0014; 0017
(2021/10/05)
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- NOVEL CYANINE COMPOUND FOR LABELING BIOMOLECULE AND PREPARATION METHOD THEREOF
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The present invention refers to biomolecule marker has a cyanine compound and number to the next formula 1 bath method are disclosed. [Formula 1] Another test, R1 , R2 , R3 , R4 , B, m and n each specification defines efined. (by machine translation)
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- A near-infrared heptamethine aminocyanine dye with a long-lived excited triplet state for photodynamic therapy
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A water-soluble near-infrared aminocyanine dye has been developed with a long triplet-state lifetime (τ = 9.16 μs in deaerated ethanol). Thereby, extremely high singlet oxygen quantum yield (ΦΔ = 0.20) and low dark cytotoxicity (IC50 = 715.4 μM) were achieved. The potential of the dye as a PDT photosensitizer was demonstrated.
- Jiao, Long,Song, Fengling,Cui, Jingnan,Peng, Xiaojun
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p. 9198 - 9201
(2018/08/24)
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- Bioorthogonally Applicable Fluorogenic Cyanine-Tetrazines for No-Wash Super-Resolution Imaging
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The synthesis, fluorogenic characterization, and labeling application of four tetrazine-quenched cyanine probes with emission maxima in the red-far red range is reported. Fluorescence of the cyanine-cores is quenched via through-bond-energy-transfer (TBET) exerted by a bioorthogonal tetrazine unit. Upon bioorthogonal labeling reaction with cyclooctyne tagged proteins, the quenching effect ceases, and thus the fluorescence reinstates, resulting in an increase in fluorescence intensity. As a rare example among indocyanines, one of our new probes was found suitable in STED-based super-resolution imaging. The applicability of this fluorogenic Tet-Cy3 probe was therefore further demonstrated in the bioorthogonal labeling of cytoskeletal protein, actin, with subsequent super-resolution microscopy (STED) imaging even under no-wash conditions.
- Knorr, Gergely,Kozma, Eszter,Schaart, Judith M.,Németh, Krisztina,T?r?k, Gy?rgy,Kele, Péter
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p. 1312 - 1318
(2018/04/23)
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- Near-infrared aminocyanine dyes with long-life triplet excited state and preparation method of dyes
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The invention provides near-infrared aminocyanine dyes with a long-life triplet excited state in the structural formula I. In the formula, X is C(CH3)2, O, S or Se; R1 and R2 are independently selected from C1-C18 alkyl or benzyl, and R3 and R4 are H, SO3R6, CO2R7 or NCS; Y is a halogen ion, ClO4, PF6, BF4, CH3COO or OTs. The heptamethine aminocyanine dye compounds I have longertriplet excited state life and higher singlet state oxygen production capability as compared with commercial dye ICG (indocyanine green) and have the potential as a near-infrared photosensitizer for photodynamic therapy.
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Paragraph 0031; 0034; 0035
(2018/05/16)
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- NOVEL CYANINE COMPOUND FOR LABELING BIOMOLECULE AND PREPARATION METHOD THEREOF
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The present invention relates to a novel cyanine compound which is represented by chemical formula 1, and is used for biomolecular labeling. The present invention further relates to a production method thereof. In the chemical formula 1, R_1, R_2, R_3, R_4, B, m, and n are the same as defined in the present specification.COPYRIGHT KIPO 2017
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- A class of amino cyanine fluorochrome and its preparation method and application
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The invention discloses amino cyanine fluorescent dyes connected with cyclopeptide [cyclo(RGD)] and a preparation method therefor and application thereof. The structural formula of the compounds is shown in I. The compounds consist of structural units III and III; the structural unit II is a derived cyanine dye structure which is water soluble fluorescent molecules, the spectrum of which has relatively great Stokes shift, and the molecules have relatively less toxicity to normal cells; the structural unit III is a cyclopeptide structure of a specific ligand of an alpha v beta 3 integrin receptor and can be used for targeted orientation. The fluorescent dyes have the double function characteristic of tumor imaging diagnosis and targeted therapy.
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- Imaging agents useful for brain and prepration method thereof
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The present invention relates to a fluorescent dye for brain imaging, which is represented by the following [Chemical Formula 1]. [Chemical Formula 1] In the [Chemical Formula 1], R_1, R_2, R_3, R_4, R_5, and n are as defined in the specification.(AA) Example 1(BB) Example 3(CC) Example 4(DD) Example 5COPYRIGHT KIPO 2017
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Paragraph 0089-0091
(2017/08/02)
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- FLUORESCENCE COMPOUNDS AND PREPARATION METHOD THEREOF
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Provided is a fluorescent compound represented by the following [Chemical Formula 1] and a method for preparing the same: wherein each of X, Y, R1, R2, R3 and n is the same as defined in the specification.
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Paragraph 0156; 0157; 0158; 0159; 0160
(2017/11/16)
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- NOVEL CYANINE COMPOUND FOR LABELING BIOMOLECULE AND PREPARATION METHOD THEREOF
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The present invention relates to a novel cyanine compound represented by chemical formula 1 for labeling a biomolecule, and to a preparation method thereof. In chemical formula 1, each of R_1, R_2, R_3, R_4, B, m, and n is the same as defined in the specification. The cyanine compound has high stability, and thus can be easily stored for a long period of time. Also, the cyanine compound does not generate byproducts after a biomolecule forms a bond with a pigment, and thus does not require a subsidiary purification process.
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- A Covalent Approach for Site-Specific RNA Labeling in Mammalian Cells
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Advances in RNA research and RNA nanotechnology depend on the ability to manipulate and probe RNA with high precision through chemical approaches, both in vitro and in mammalian cells. However, covalent RNA labeling methods with scope and versatility comp
- Li, Fahui,Dong, Jianshu,Hu, Xiaosong,Gong, Weimin,Li, Jiasong,Shen, Jing,Tian, Huifang,Wang, Jiangyun
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supporting information
p. 4597 - 4602
(2015/04/14)
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- Cyanine compound for labeling biomolecule and preparation method thereof
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Disclosed are a novel cyanine compound, represented by the following Formula 1, for labeling biomolecules, and a method for preparing the same. wherein R1, R2, R3, R4, B, m and n are defined as above.
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- Synthesis of quaternary heterocyclic salts
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The microwave synthesis of twenty quaternary ammonium salts is described. The syntheses feature comparable yields to conventional synthetic methods reported in the current literature with reduced reaction times and the absence of solvent or minimal solvent.
- Winstead, Angela J.,Nyambura, Grace,Matthews, Rachael,Toney, Deveine,Oyaghire, Stanley
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p. 14306 - 14319
(2014/01/06)
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- DYES FOR LABELLING MOLECULAR LIGANDS
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Cyanine dyes with improved fluorescence intensity and photostability.
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Paragraph 00139; 00140
(2013/04/10)
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- Near-infrared heptamethine cyanine dyes. Synthesis, spectroscopic characterization, thermal properties and photostability
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Near-infrared heptamethine cyanine dyes were synthesized without substitution to the polyene chain but with various substituents on the indoleninium fragments. To develop indotricarbocyanine dyes with improved photochemical characteristics, we studied the
- Shershov, Valeriy E.,Spitsyn, Maksim A.,Kuznetsova, Viktoriya E.,Timofeev, Edward N.,Ivashkina, Olga A.,Abramov, Ivan S.,Nasedkina, Tatyana V.,Zasedatelev, Alexander S.,Chudinov, Alexander V.
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p. 353 - 360
(2013/04/23)
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- INDICYANINE DYES AND THE DERIVATIVES THEREOF FOR ANALYSING BIOLOGICAL MICROMOLECULES
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The invention relates to two series of synthesised indolenine pentamethine dyes (37 compounds) comprising a reactive group in the third and fifth positions of the indolenine cycle, respectively. A method for producing, extracting and purifying the dyes and the precursors thereof, consisting in determining the absorption and fluorescence maxima, molecular extinction factors, fluorescence quantum yields, relative fluorescence effectiveness at excitation on a wavelength of 635 nm (detection of 670 nm) and 655 nm (detection of 690 nm), relative light stability and comparative sensitivity of detection of marked oligonucleotides on biochips of the claimed dyes, is disclosed. The use of the claimed dyes in the form of fluorescent marks for oligonucleotide and protein chips is also disclosed.
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Page/Page column 51-52
(2010/04/23)
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- Synthesis of PEG derivatives bearing aminophenyl and their application for liquid-phase synthesis of water-soluble unsymmetrical cyanine dyes
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The synthesis and NMR characterization of soluble PEG-supported polymers were described, and their subsequent application for liquid-phase synthesis of water-soluble cyanine dyes was also studied. Nucleophilic substitution of tosylation of PEG 16 with 1,3-bis(4-nitrophenoxy)-2-propanol 15 under basic conditions, followed by nitro group reduction, gave PEG-bound aminophenyl 18. Another PEG-bound aminophenyl 28 was prepared by condensation reaction of PEG-bound pentaerythritol 25 and 4-aminobenzoic acid followed by the cleavage of BOC group. Subsequent loading and activation of sulfoindoleninium to PEG derivatives 18 or 28 were achieved via simple strategies. Cyanine dyes were released by the attack of heterocyclic carbon nucleophile and the cleavage of PEG-bound hemicyanine without the chromatographic separation. The efficient, facile, and practical approaches appear to be robust and versatile strategies to deliver not only indocyanine dyes but also benzoindocyanine dyes.
- Jiang, Lin-Ling,Li, Bao-Lin,Lv, Feng-Ting,Dou, Li-Fang,Wang, Liu-Chang
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scheme or table
p. 5257 - 5264
(2009/12/01)
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- An efficient approach to the synthesis of water-soluble cyanine dyes using poly(ethylene glycol) as a soluble support
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An efficient synthesis approach to unsymmetrical water-soluble cyanine dyes has been established. Loading and activation of sulfoindolenium to poly(ethylene glycol) (PEG) have been achieved via a simple strategy. Cyanine dyes are released by the attack of heterocyclic carbon nucleophile and the cleavage of PEG-bound hemicyanine. The efficient approach delivers cyanine dyes in high purity without the nontrivial chromatographic separation.
- Jiang, Lin-Ling,Dou, Li-Fang,Li, Bao-Lin
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p. 5825 - 5829
(2008/02/09)
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- PH sensitive cyanine dyes as reactive fluorescent reagents
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The present invention provides pH sensitive cyanine dyes having the structure (I) wherein X and Y are independently selected from >C(C1 - C4alkyl)2, sulfur and oxygen; R1 and R2 are independently selected from H, CH2NH2, SO3-, phosphate, phosphonate, quaternary ammonium, (CH2)qCOOH, NCS, CH2NH-COR7, where R7 is C1 - C20 straight or branched alkyl and (CH2)qCOOH where q is an integer from 0 - 10; R3 is H or -L-P where L is selected from C1 - C20 straight or branched alkyl optionally containing 0, 1 or 2 unsaturated groups selected from alkenyl and alkynyl, and P is selected from a reactive group, H, C1 - C20 straight or branched alkyl, SO3-, NH2, quaternary ammonium, CH2NH-COR8, where R8 is C1 - C20 straight or branched alkyl, -(CH2)mCOOH, NHR9 where R9 is C1 - C20 straight or branched alkyl; n is an integer from 0 - 3; p and r are independently 0, 1, 2, 3 or 4 and where p and/or r is greater than 1, each R1 and each R2 may be different, and m is an integer from 1 - 10; and salts and protonated derivatives thereof and methods of using same as fluorescent reagents.
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- Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
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This invention provides for labeling reagents, labeled targets and processes for preparing labeling reagents. The labeling reagents can take the form of cyanine dyes, xanthene dyes, porphyrin dyes, coumarin dyes or composite dyes. These labeling reagents are useful for labeling probes or targets, including nucleic acids and proteins. These reagents can be usefully applied to protein and nucleic acid probe based assays. They are also applicable to real-time detection processes.
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- Squarate dyes and their use in fluorescent sequencing method
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Novel squarate dyes are described, and adducts of these dyes with biologically significant chemical species such as nucleosides or nucleotides. The adducts have useful properties for fluorescent nucleic acid sequencing methods.
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