- The existence of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA in mammals
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We developed a novel strategy by oxidation-derivatization combined mass spectrometry analysis for the determination of 5-hydroxymethylcytosine and 5-formylcytosine in both DNA and RNA. We reported the presence of 5-formylcytosine in RNA of mammals and found that ascorbic acid and hydroquinone can increase the oxidation of 5-methylcytosine to 5-hydroxymethylcytosine in DNA and RNA.
- Zhang, Hao-Ying,Xiong, Jun,Qi, Bao-Ling,Feng, Yu-Qi,Yuan, Bi-Feng
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- A Chemical Photo-Oxidation of 5-Methyl Cytidines
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A new approach for the oxidation of 5-methyl cytidine modification in nucleic acids to 5-formyl cytidine is reported. This method displays excellent chemo-/bio- selectivity, from which the methyl group in cytidine nucleosides could be selectively oxidized to biologically important formyl functionality without interfering with other cellular biomolecules. (Figure presented.).
- Jin, Xiao-Yang,Wang, Rui-Li,Xie, Li-Jun,Kong, De-Long,Liu, Li,Cheng, Liang
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- SYNTHESIS AND STRUCTURE OF HIGH POTENCY RNA THERAPEUTICS
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This invention provides expressible polynucleotides, which can express a target protein or polypeptide. Synthetic mRNA constructs for producing a protein or polypeptide can contain one or more 5′ UTRs, where a 5′ UTR may be expressed by a gene of a plant. In some embodiments, a 5′ UTR may be expressed by a gene of a member of Arabidopsis genus. The synthetic mRNA constructs can be used as pharmaceutical agents for expressing a target protein or polypeptide in vivo.
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- Facile methods for the synthesis of 5-formylcytidine
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5′-O-Protected 5-methylcytidine 3 was oxidized with Na2S2O8 to give a mixture of the corresponding 5-(hydroxymethyl)- and 5-formylcytidine derivatives, 4 and 5. The hydroxymethyl group of 4 was further oxidized to a formyl group by treatment with ceric(IV) ammonium nitrate (CAN). Alternatively, 2′,3′,5′-O-protected 5-(hydroxymethyl)cytidine 10 was directly oxidized with CAN to give the desired 5-formylcytidine derivative 11. After removal of the protecting groups in each intermediate, 5-formylcytidine (6) was obtained in good yield.
- Abdel Rahman, Adel A.-H.,Wada, Takeshi,Saigo, Kazuhiko
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p. 1061 - 1063
(2007/10/03)
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