- Method for assaying the sod activity by using a self-oxidizable compound necessary for its implementation, self-oxidizable compounds and preparation thereof
-
The method for assaying the SOD (super oxide dismutase) activity in liquid medium is based on the activation of self-oxidization, by SOD activity, of a reactive agent having the general formula (I) wherein either n is 1 or 2, R1 is --OR4 or --NR5 R6 ; R2 is H, --OR4, alkyl (1-6C), --CH2 -- or --CH2 --CH2 --, to form a ring by binding to the phenyl substituent, at meta with respect to R1 ; and R3 is H, alkyl (1-6C) or --OR4 (if R2 is different from --OR4); with R4 being H or alkyl (1-6C); R5 being H, alkyl (1-6C), --CH2 COOH, --C6 H5 COOH or --C6 H5 SO3 H; and R6 is H, alkyl (1-6C) or --CH2 COOH; or n is 1, R1 is --OR4, R2 is --CH2 --O--, in order to form a ring by bonding of O with the phenyl substituent, at meta with respect to R1 ; and R3 is H or --OR4. Application to assaying the SOD activity in a sample, specially a biological sample, particularly by a single measurement and one calibrating curve.
- -
-
-