Chemoenzymic synthesis of P(α)-methyl deoxynucleoside triphosphates
5'-O-(methylphosphonyl)-N-(phenylacetyl)-2'-deoxycytidine, deoxyadenosine and deoxyguanosine were pyrophosphorylated and the resulting N-protected P(α)-methyl nucleoside triphosphates were deblocked by treatment with penicillin amidase at pH 7.8, 25°C to give P(α)-methyl nucleoside triphosphates.