- Highly Selective Fluorescent Probe Based on Hydroxylation of Phenylboronic Acid Pinacol Ester for Detection of Tyrosinase in Cells
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The detection of tyrosinase, a biomarker for melanoma, is of great significance. Herein, a fluorescent tyrosinase probe, with resorufin as the fluorophore and m-tolylboronic acid pinacol ester as the receptor, is proposed. The response relies on the tyrosinase-catalyzed hydroxylation of phenylboronic acid pinacol ester at an adjacent position followed by 1,6-rearrangement elimination to release resorufin. This probe well quantifies tyrosinase in the range from 1 to 100 U mL-1 with a detection limit of 0.5 U mL-1. Importantly, the probe exhibits high selectivity for tyrosinase over other biological substances including reactive oxygen species. In addition, it is successfully applied to the imaging of tyrosinase in cells. This probe provides a novel platform for selective detection of tyrosinase in biosystems.
- Li, Huihui,Liu, Wei,Zhang, Fengyuan,Zhu, Xinyue,Huang, Liqiu,Zhang, Haixia
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- Selective ratiometric detection of H2O2 in water and in living cells with boronobenzo[b]quinolizinium derivatives
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Boronobenzo[b]quinolizinium derivatives exhibit several favorable properties for the fluorimetric detection of hydrogen peroxide, namely quantitative transformation to a product whose emission maximum is well separated from the one of the substrate, water solubility, and the ability to operate in living cells. This journal is the Partner Organisations 2014.
- Bortolozzi, Roberta,Gradowski, Sebastian Von,Ihmels, Heiko,Sch?fer, Katy,Viola, Giampietro
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supporting information
p. 8242 - 8245
(2014/07/21)
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- PYRAZOLE COMPOUND AND PHARMACEUTICAL USE THEREOF
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The present invention provides a pyrazole compound of the following general Formula [1b] having SGLT1 inhibitory activity, or a pharmaceutically acceptable salt thereof, a pharmaceutical composition comprising the same, and its pharmaceutical use wherein each symbol is the same as defined in the description
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Paragraph 0960; 0961; 0962
(2013/04/13)
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- Inhibition of RANTES/CCR1-mediated chemotaxis by cosalane and related compounds
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The anti-HIV agent cosalane and several of its analogues inhibited RANTES-induced migration of human monocytes, but they did not inhibit migration induced by MIP1α or MIP1β. RANTES-induced migration of single receptor CCR1-HEK transfectants was also inhib
- Howard,Dong, Hui Fang,Oppenheim, Joost J.,Insaf, Shabana,Santhosh, Kalpathy C.,Paul, Gitendra,Cushman, Mark
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