- Modification of mtt assay for precision and repeatability and its mechanistic implication
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The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is an attractive method for antibiotics screening. However, a severe deviation will occur when a strong acidic sodium dodecyl sulfate is used as a lysis buffer in MTT assay and n
- Wang, Xu-Dong,Deng, Rui-Cheng,Liu, Yao,Li, Biao,Huang, Shen,Ouyang, Hui,Xiao, Zhu-Ping
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- Studies on some metal complexes of a quinoxaline?based unsymmetrical ONNO donor ligand: Synthesis, spectral characterization, thermal, in vitro biological and DFT studies
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Abstract: Co(II), Ni(II), Cu(II) and Zn(II) complexes of an unsymmetrical tetradentate ONNO donor ligand, 3-(-(3-(-1-(2-hydroxyphenyl)ethylideneamino)propylimino)methyl)quinoxalin-2(1H)-one (L) have been synthesized and characterized by elemental analysis, molar conductance, magnetic susceptibility measurements, FTIR, UV–Vis., mass and 1H NMR spectral studies. ESR spectra of the Cu(II) complex under room (300?K) and liquid nitrogen temperature (77?K) were also recorded. Thermal behavior of the newly synthesized ligand and its metal complexes was assessed by TG–DTG analysis. All the complexes are found to be mononuclear. Crystallinity, average grain size and unit cell parameters were determined from powder X-ray diffraction study. Electrochemical behavior of the compounds was examined by cyclic voltammetry technique. The in vitro antimicrobial screening of the unsymmetrical ligand and corresponding metal chelates was tested against some bacterial strains (E. coli, K. pneumoniae, S. pneumoniae and S. aureus) and fungal strains (A. niger, A. flavus, P. chrysogenum and R. stolonifer). The in vitro antioxidant and anticancer activities of the compounds were also evaluated. DFT studies were done to optimize the structure of the compounds and to calculate nonlinear optical properties of the compounds. Graphical Abstract: [Figure not available: see fulltext.]
- Dhanaraj, C. Justin,Johnson, Jijo
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p. 1845 - 1862
(2017/02/10)
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