- Chemical bromination of phenol red by hydrogen peroxide is possible in the absence of haloperoxidases
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The dye phenol red (phenolsulphonphthalein) was chemically brominated to bromophenol blue (3',3',5',5'-tetrabromophenolsulphonphthalein) directly by hydrogen peroxide (20 mM or higher) under acidic pH (0.5-4.5) and moderate temperature (30°C). Since this bromination reaction takes place in the absence of any chemical or biological catalyst, the results represent an important warning concerning a commonly used assay for detecting or screening haloperoxidases. Bromophenol red (5',5'-dibromophenolsulphonphthalein) and another unidentified compound were observed as temporary intermediates. Incubation of the reaction mixture with KCl instead of NaBr did not yield any chlorinated products under the same conditions.
- Terron, Maria C.,Verhagen, Frank J.M.,Franssen, Maurice C.R.,Field, Jim A.
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p. 1445 - 1452
(2007/10/03)
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