- Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol-phosphate dehydrogenase
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D-Arabinitol 1-phosphate (Ara-ol1-P), a substrate for d-arabinitol- phosphate dehydrogenase (APDH), was chemically synthesized from d-arabinonic acid in five steps (O-acetylation, chlorination, reduction, phosphorylation, and de-O-acetylation). Ara-ol1-P was used as a substrate for the characterization of APDH from Bacillus halodurans. APDH converts Ara-ol1-P to xylulose 5-phosphate in the oxidative reaction; both NAD+ and NADP+ were accepted as co-factors. Kinetic parameters for the oxidative and reductive reactions are consistent with a ternary complex mechanism.
- Soroka, Nikolai V.,Kulminskaya, Anna A.,Eneyskaya, Elena V.,Shabalin, Konstantin A.,Uffimtcev, Andrei V.,Povelainen, Mira,Miasnikov, Andrei N.,Neustroev, Kirill N.
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p. 539 - 546
(2007/10/03)
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