- Temperature-sensitive fluorescent probe as well as preparation method and application thereof
-
The invention discloses a temperature-sensitive fluorescent probe as well as a preparation method and application thereof, and belongs to the field of chemical biology. The molecular formula of the temperature-sensitive fluorescent probe is C33H42ClN3O, and the molecular weight of the temperature-sensitive fluorescent probe is 531.3016; wherein the preparation method of the temperature-sensitive fluorescent probe comprises the following steps: preparing ester group protected rhodamine B; preparing hydroxyl modified rhodamine B; preparing chloro rhodamine B; and preparing the temperature-sensitive fluorescent probe. The temperature-sensitive fluorescent probe RhBIV prepared by the invention has good temperature sensitivity, is targeted to cell mitochondria, and can be used for observing the temperature change of the mitochondria on living cells and living small animals in real time. The probe can be used for screening drugs for regulating and controlling energy metabolism and monitoring the temperature change of visceral organs in real time at a living animal level; therefore, the novel detection method is provided for related pharmacodynamic evaluation and has a good application prospect.
- -
-
Paragraph 0035; 0051-0052
(2021/09/26)
-
- Salt and colored curable composition
-
The invention provides a salt. The salt contains at least one anion selected from anodes respectively represented by formulas ( I ), (II), (III) and (IV) and cation having xanthene skeleton, wherein in the formula (I), X1 and X2 respectively represent flu
- -
-
Paragraph 0433-0435
(2017/01/31)
-
- 1H and 13C NMR spectra of commercial rhodamine ester derivatives
-
Ethyl and methyl esters of commercial rhodamines B, 19, 101 and 110 and propyl and butyl esters of commercial rhodamine B were synthesized and isolated with different counterions (yields 70-98%). The 1H and 13C NMR spectral data for these compounds were fully assigned by a combination of one-and two-dimensional experiments. The Fourier transform IR and UV-visible spectra were also recorded and the main bands were identified. Copyright
- Ramos,Vilhena,Santos,Almeida
-
p. 475 - 478
(2007/10/03)
-