- Exploiting long-lived molecular fluorescence
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Fluorophores based on the azo chromophore 2,3-diazabicyclo[2.2.2]oct-2-ene, referred to as fluorazophores, display an exceedingly long fluorescence lifetime. Besides the use in time-resolved screening assays, where the long-lived fluorescence can be time-gated, thereby reproving the signal to background ratio, a distinct application of fluorazophores lies in the area of biopolymer dynamics. For this purpose, one chain end is labeled with a fluorazophore and the other one with an efficient fluorescence quencher. The fluorescence lifetime of the probe/quencher-labeled peptide then reflects the kinetics of intramolecular end-to-end collision. Applications to polypeptides are described and control experiments which establish the nature of the quenching mechanism as a diffusive process requiring intimate probe/quencher contact are described.
- Nau, Werner M.,Huang, Fang,Wang, Xiaojuan,Bakirci, Huseyin,Gramlich, Gabriela,Marquez, Cesar
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p. 161 - 167
(2007/10/03)
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- A fluorescence-based method for direct measurement of submicrosecond intramolecular contact formation in biopolymers: An exploratory study with polypeptides
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A fluorescent amino acid derivative (Fmoc-DBO) has been synthesized, which contains 2,3 diazabicyclo[2.2.2]oct-2-ene (DBO) as a small, hydrophilic fluorophore with an extremely long fluorescence lifetime (325 ns in H2O and 505 ns in D2/su
- Hudgins, Robert R.,Huang, Fang,Gramlich, Gabriela,Nau, Werner M.
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p. 556 - 564
(2007/10/03)
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