- S-2-Hydroxyacylglutathione-Derivatives: Enzymatic Preparation, Purification and Characterisation
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S-2-Hydroxyacylglutathione derivatives have been prepared by enzymatic synthesis from α-oxoaldehydes and reduced glutathione in the presence of glyoxalase I.S-D-Lactoylglutathione, S-D-mandelylglutathione, S-glycolylglutathione and S-L-glyceroylglutathione were prepared from methylglyoxal, phenylglyoxal, glyoxal and hydroxypyruvaldehyde, respectively.They were purified by ion exchange chromatography on Dowex 1 on a gram scale.Analytical data and re-evaluated extinction coefficients for these compounds are presented.The method described provides a reliable, large-scale procedure for the preparation and purification of S-acylglutathiones of increasing biological and pharmacological interest.
- Clelland, James D.,Thornalley, Paul J.
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p. 3009 - 3016
(2007/10/02)
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- Photosensitized NAD(P)H Regeneration Systems; Application in the Reduction of Butan-2-one, Pyruvic, and Acetoacetic Acids and in the Reductive Amination of Pyruvic and Oxoglutaric Acid to Amino Acid
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The photosensitized formation of NAD(P)H by enzyme-catalysed processes has been accomplished.With Ru(bpy)32+ as sensitizer, methyl viologen, MV2+ as primary electron acceptor, and (NH4)3EDTA or 2- mercaptoethanol, NADPH is formed in the presence of ferredoxin NADP+-reductase as enzyme catalyst.Zinc(II)meso-tetramethylpyridiniumporphyrin, ZnTMPy4+ is used as sensitizer for the photoinduced production of NADH using the same components and lipoamide dehydrogenase as enzyme catalyst.The photoinduced NAD(P)H regeneration systems have been coupled to secondary enzyme-catalysed processes such as the reduction of butan-2-one to butan-2-ol, pyruvic acid to lactic acid, acetoacetic acid to β-hydroxybutyric acid, as well as to the reductive amination of pyruvic acid to alanine and of α-oxoglutaric acid to glutamine acid.The products exhibit high optical purity and the enzymes and the coenzymes show high turnover numbers and stability.
- Mandler, Daniel,Willner, Itamar
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p. 805 - 812
(2007/10/02)
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