Ultrafast extraction of proteins from tissues using desorption by impulsive vibrational excitation
A picosecond IR laser (PIRL) can be used to blast proteins out of tissues through desorption by impulsive excitation (DIVE) of intramolecular vibrational states of water molecules in the cell in less than a millisecond. With PIRL-DIVE proteins covering a range of a few kDa up to several MDa are extracted in high quantities compared to conventional approaches. The chemical composition of extracted proteins remains unaltered and even enzymatic activities are maintained.
Kwiatkowski, Marcel,Wurlitzer, Marcus,Omidi, Maryam,Nimer, Refat,Horst, Andrea,Schlüter, Hartmut,Ren, Ling,Kruber, Sebastian,Robertson, Wesley D.,Miller, R. J. Dwayne
supporting information
p. 285 - 288
(2015/07/02)
ACTION ON PEPTIDES BY WHEAT CARBOXYPEPTIDASE
A kinetic analysis has been performed with purified wheat carboxypeptidase by the use of N-acyl dipeptides, Z-Gly-Pro-Leu-Gly (Z=benzyloxycarbonyl), angiotensin II and bradykinin.The values of kcat were dramatically influenced by amino acid residues occupying the penultimate position from the carbonyl terminus of substrates.The structure of the substrate did not appreciably affect the Km values. Key Word Index - Triticum aestivum; Gramineae; wheat; carboxypeptidase; peptides; kinetic parameters.
Umetsu, H.,Ichishima, E.
p. 591 - 592
(2007/10/02)
Synthesis of peptides with the solid phase method. II. Octapeptide analogues of angiotensin II
-
Park,Choi,Rioux,Regoli
p. 113 - 119
(2007/10/09)
More Articles about upstream products of 51833-78-4