- Quantitative determination of SH groups using 19F NMR spectroscopy and disulfide of 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid
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A new method of measurement of thiol concentration by 19F NMR spectroscopy is developed. The method is based on the detection of products of the exchange reaction of thiols with a newly synthesized fluorinated disulfide, 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid (BSSB). A significant broadening of the 19F NMR signal of BSSB in the presence of thiols was observed and attributed to the exchange reaction between the parent disulfide and 2,3,5,6-tetrafluoro-4-mercaptobenzoic acid. The rate constant for this reaction was found to be equal to (63 ± 11) × 103 M-1 s-1 at pH 7.0. The method was applied for the measurement of concentration of glutathione and albumin in rat blood. Copyright
- Potapenko, Dmitrii I.,Bagryanskaya, Elena G.,Grigoriev, Igor A.,Maksimov, Aleksander M.,Reznikov, Vladimir A.,Platonov, Vyacheslav E.,Clanton, Thomas L.,Khramtsov, Valery V.
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- Synthesis and reactions of 4-sulpho-2,3,5,6-tetrafluorobenzoic acid
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4-Sulpho-2,3,5,6-tetrafluorobenzoic acid (I) has been synthesised from pentafluorobenzoic acid via the oxidation of 4-mercapto-tetrafluorobenzoic acid.Reaction of I with DMF/SOCl2 gave 1,4-ClC6F4COCl (IIA) which was converted to 1,4-ClC6F4COF (IIB) by flu
- Fielding, H. C.,Shirley, I. M.
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