Candida tropicalis CE017: A new Brazilian enzymatic source for the bioreduction of aromatic prochiral ketones
The reactivity and stereoselectivity showed by a new strain of Candida tropicalis in the reduction of prochiral ketones have been compared with the ones previously attained in our laboratory using microorganisms from the Brazilian biodiversity. In this manner, Candida tropicalis has demonstrated its versatility as stereoselective agent in the bioreduction of a series of aromatic ketones. These prochiral compounds were converted into their corresponding optically alcohols with moderate to excellent stereopreference depending on the substrate structure. Among ketones tested, nitroacetophenones were enzymatically reduced to enantiopure (S)-alcohol with complete conversion.
Vieira, Gizelle A. B.,De Freitas Araujo, Daniel M.,Lemos, Telma L. G.,De Mattos, Marcos Carlos,Da Conceic?a?o F. De Oliveira, Maria,Melo, Va?nia M. M.,De Gonzalo, Gonzalo,Gotor-Ferna?ndez, Vicente,Gotor, Vicente
experimental part
p. 1509 - 1516
(2010/11/04)
Reduction processes biocatalyzed by Vigna unguiculata
Whole cells from the Brazilian beans feijao de corda (Vigna unguiculata) have been employed as biocatalysts in different bioreduction processes. Good to excellent selectivities can be obtained in the reduction of aromatic and aliphatic ketones, as well as β-ketoesters, depending on the conversions and the chemoselectivity on the substrate structure. This biocatalyst was also able to reduce the nitro moiety of different aromatic nitro compounds, showing as well enoate reductase activity, and chemoselectively catalyzing the double bond reduction of 4-phenyl-3-buten-2-one with moderate conversion.
Bizerra, Ayla M.C.,Gonzalo, Gonzalo de,Lavandera, Ivan,Gotor-Fernandez, Vicente,de Mattos, Marcos Carlos,de Oliveira, Maria da Conceicao F.,Lemos, Telma L.G.,Gotor, Vicente
experimental part
p. 566 - 570
(2010/08/06)
Lentinus strigellus: a new versatile stereoselective biocatalyst for the bioreduction of prochiral ketones
Growing cells of the basiodiomycete Lentinus strigellus in potato-dextrose broth have been used for the first time as a biocatalyst in the stereoselective reduction of aromatic and aliphatic ketones. Most of the aromatic ketones were converted into the corresponding optically active alcohols in up to >99% enantiomeric excess under very mild reaction conditions. Among the aliphatic ketones tested, 2-octanone was enzymatically reduced by this microorganism to enantiopure (S)-2-octanol with almost complete conversion.
Barros-Filho, Bartholomeu A.,de Oliveira, Maria da Conceicao F.,Lemos, Telma L.G.,de Mattos, Marcos C.,Gonzalo, Gonzalo de,Gotor-Fernandez, Vicente,Gotor, Vicente
experimental part
p. 1057 - 1061
(2009/10/02)
Tandem concurrent processes: One-pot single-catalyst biohydrogen transfer for the simultaneous preparation of enantiopure secondary alcohols