- A mitochondria-targeting fluorescent Fe3+ probe and its application in labile Fe3+ monitoring via imaging and flow cytometry
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The biogenesis of heme and iron-sulfur cluster in mitochondria is closely associated with the intracellular iron homeostasis. In this study, a mitochondria-targeting probe with the reversible turn-on fluorescent response to Fe3+ was developed for mitochondrial labile Fe3+ monitoring by modifying spirolactam rhodamine with a chelator. With this probe, the mitochondrial labile Fe3+ fluctuation in adherent cells upon ferric citrate incubation was visualzied via confocal imaging, and the flow cytometric assay for mitochondrial Fe3+ in suspension cells, which is difficult to be monitored via confocal imaging, was also developed. Moreover, the labile Fe3+ drop in mitochondria of K562 cells undergoing the DMSO-stimulated erythroid differentiation was observed for the first time.
- Zhu, Chengcheng,Wang, Mengjia,Qiu, Lin,Hao, Shuangying,Li, Kuanyu,Guo, Zijian,He, Weijiang
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- Reversible FRET Fluorescent Probe for Ratiometric Tracking of Endogenous Fe3+in Ferroptosis
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A reversible fluorescent probe DRhFe is devised by linking spirolactam rhodamine and dansylamide through an Fe3+ ionophore N2-hydroxyethyldiethylenetriamine, where Fe3+ chelation generating rhodamine B can switch intramolecular fluorescence resonance energy transfer for ratiometric Fe3+ sensing. Probe DRhFe exhibits an Fe3+-specific emission shift from 483 to 576 nm, and this ratiometric response has been successfully applied to monitor labile Fe3+ fluctuations in cells undergoing ferroptosis.
- Gao, Jing,He, Yueqin,Chen, Yuncong,Song, Dongfan,Zhang, Yuming,Qi, Fen,Guo, Zijian,He, Weijiang
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- A turn-on fluorescent Fe3+ sensor derived from an anthracene-bearing bisdiene macrocycle and its intracellular imaging application
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Integrating N2-hydroxyethyldiethylenetriamine with anthracene gives a [2+2] macrocycle fluorescent sensor. This sensor displays an instant/reversible turn-on response specific to Fe3+, which allows facile visualization of the Fe3+/Fe2+ transition and intracellular Fe3+ imaging. the Partner Organisations 2014.
- Qiu, Lin,Zhu, Chengcheng,Chen, Huachao,Hu, Ming,He, Weijiang,Guo, Zijian
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supporting information
p. 4631 - 4634
(2014/05/06)
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- First synthesis of P-chirogenic prophosphatranes
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Syntheses are reported for the novel P-chirogenic bicyclic prophosphatranes P(RNCH2CH2)2N(OCH2CH2) - (8) in which the two R groups [i.e., 1-methylenenaphthyl and 1,2-methoxybenzyl (8)] and in its corresponding phosphine oxide (9) are different. Also synthesized was the transannulated protonated phosphatrane cation in the salt [HP(RNCH2CH2)3N]Cl in which the three R groups [i.e., 1-methylenenaphthyl, 1,2-methoxybenzyl, and (S)-1-phenethyl (14)] are different, and also in its corresponding deprotonated prophosphatrane form P(RNCH2CH2)3N (15). Good analytical resolution of racemic 9 is reported, whereas only partial resolution was achieved for diastereomeric 14.
- Zhou, Yibo,Armstrong, Daniel W.,Zhang, Ying,Verkade, John G.
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supporting information; experimental part
p. 1545 - 1548
(2011/04/26)
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