- 3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents
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Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.
- Busse, Madleen,Marciniszyn, Jaroslaw P.,Ferstl, Simone,Kimm, Melanie A.,Pfeiffer, Franz,Gulder, Tanja
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supporting information
p. 4561 - 4566
(2021/02/12)
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- Novel β-cyclodextrin-eosin conjugates
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Eosin B (EoB) and eosin Y (EoY), two xanthene dye derivatives with photosensitizing ability were prepared in high purity through an improved synthetic route. The dyes were grafted to a 6-monoamino-β-cyclodextrin scaffold under mild reaction conditions through a stable amide linkage using the coupling agent 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride. The molecular conjugates, well soluble in aqueous medium, were extensively characterized by 1D and 2D NMR spectroscopy and mass spectrometry. Preliminary spectroscopic investigations showed that the β-cyclodextrin-EoY conjugate retains both the fluorescence properties and the capability to photogenerate singlet oxygen of the unbound chromophore. In contrast, the corresponding β-cyclodextrin-EoB conjugate did not show either relevant emission or photosensitizing activity probably due to aggregation in aqueous medium, which precludes any response to light excitation.
- Benkovics, Gábor,Afonso, Damien,Darcsi, András,Béni, Szabolcs,Conoci, Sabrina,Fenyvesi, éva,Szente, Lajos,Malanga, Milo,Sortino, Salvatore
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supporting information
p. 543 - 551
(2017/03/29)
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