- Conjugated polyelectrolyte based real-time fluorescence assay for phospholipase C
-
A fluorescence turnoff assay for phospholipase C (PLC) from Clostridium perfringens is developed based on the reversible interaction between the natural substrate, phosphatidylcholine, and a fluorescent, water-soluble conjugated polyelectrolyte (CPE). The fluorescence intensity of the CPE in water is increased substantially by the addition of the phospholipid due to the formation of a CPE-lipid complex. Incubation of the CPE-lipid complex with the enzyme PLC causes the fluorescence intensity to decrease (turnoff sensor); the response arises due to PLC-catalyzed hydrolysis of the phosphatidylcholine, which effectively disrupts the CPE-lipid complex. The PLC assay operates with phospholipid substrate concentrations in the micromolar range, and the analytical detection limit for PLC is 2+ and inhibition by EDTA and fluoride ion are demonstrated using the optimized sensor.
- Liu, Yan,Ogawa, Katsu,Schanze, Kirk S.
-
p. 150 - 158
(2008/09/17)
-
- Optically active oxiranes. Synthesis of PAF (Platelet Aggregating Factor)
-
A number of epoxides bearing no function in the α-position have been converted into β-hydroxy sulfonium salts.Association with an otically active acid (dibenzoyltartaric) allowed the resolution.This method has been used to prepare the optically active glycidol octadecyl ether which was converted into (-) PAF. Key words: oxiranes / resolution / PAF synthesis
- Cimetiere, B,Jacob, L,Julia, M
-
p. 926 - 938
(2007/10/02)
-