- Enzymatic synthesis of mannobioses and mannotrioses by reverse hydrolysis using α-mannosidase from Aspergillus niger
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Various manno-oligosaccharides including α-D-man-(12)-D-man and α-D-man-(12)-α-D-man-(12)-D-man were formed when a highly concentrated mannose solution was incubated in the presence of α-mannosidase from Aspergillus niger. α-D-Man-(12)-D-man and α-D-man-(12)-α-D-man-(12)-D-man were isolated by activated carbon chromatography followed by high performance liquid chromatography using an amino-silica column.In addition to the above oligosaccharides, α-D-man-(13)-D-man, α-D-man-(16)-D-man, and α-D-man-(12)-α-D-man-(16)-D-man were also isolated. Keywords: Mannobioses; Mannotrioses; α-Mannosidase; Aspergillus niger
- Ajisaka, Katsumi,Matsuo, Ichiro,Isomura, Megumi,Fujimoto, Hiroshi,Shirakabe, Mayumi,Okawa, Mitsuyo
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p. 123 - 130
(2007/10/02)
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- ACETOLYSIS OF Leukonostoc mesenteroides NRRL B-1299 DEXTRAN. ISOLATION AND CHARACTERIZATION OF OLIGOSACCHARIDES CONTAINING SECONDARY LINKAGES FROM THE BORATE-SOLUBLE FRACTION
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Fractionation of the deacetylated acetolyzate of the borate-soluble fraction of the dextran elaborated by Leuconostoc mesenteroides MRRL B-1299 gave, after chromatography on charcoal-Celite, preparative paper-chromatography, and paper electrophoresis, four trisaccharide fractions and four tetrasaccharide fractions.The isolated oligosaccharides were characterized by their paper-chromatographic mobility, examination of partial acid-hydrolyzates of the oligosaccharides and their corresponding alditols, and methylation analysis.These oligosaccharides were shown to be (a) kojitriose (1), (b) isomaltotriose (2), (c) a mixture of 2-O-α-isomaltosyl-D-glucose (3), 21-O-α-D-glucosylisomaltose (4), and 2-O-α-nigerosyl-D-glucose (5), (d) 6-O-α-kojibiosyl-D-glucose (6), (e) isomaltotetraose (7), (f) a mixture of 2-O-α-isomaltotriosyl-D-glucose (8) and 21-O-α-D-glycosylisomaltotriose (9), (g) 6-O-α-kojitriosyl-D-glucose (10), and (h) 63-O-α-D-glucosylkojitriose (11), respectively.Some of these oligosaccharides are newly isolated and characterized.
- Watanabe, Toshiyuki,Chiba, Michiko,Matsuda, Yutaka,Sakurai, Fukuko,Kobayashi, Mikihiko,Matsuda, Kazuo
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p. 119 - 128
(2007/10/02)
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