Design and synthesis of chromogenic thiopeptolide substrates as MetAPs active site probes
Twenty one chromogenic thiopeptolide substrates were designed and synthesized as the active site probes and analyzed with each S1 site of mutant residues and enzymes of wild-type MetAP1s. The preliminary enzymatic experiments indicate that cysteine 70 or 202, at either Escherichia coli or human MetAP1, played a crucial role in the methionine hydrolysis.
Get Best Price for736170-76-6(2S)-3-tert-butoxy-2-{(2R)-2-[(2S)-2-tert-butoxy-carbonylamino-4-methylsulfanyl-butyrylsulfanyl]-3-phenyl-propionylamino}-propionic acid tert-butyl ester