- eoxyribonucleic Acids and Related Compounds. VIII. Solid-Phase Synthesis of Deoxyribooligonucleotides with 3'-Modification by Elongation in the 3'-Direction
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Four dodecanucleotides dATGTTTCCCTCTpOAr (12a), dATGATGTGGTATpOAr (12b), dATGTTGCCCGTTpOAr (12c) and dATGTGTCTGCGGpOAr (12d) having the 3'-(o-chlorophenyl)phosphate moiety have been synthesized on a polymer support by elongation in the 3'-direction.The 5'
- Ohtsuka, Eiko,Taniyama, Yoshio,iWai, Shigenori,Yoshida, Tadao,Ikehara, Morio
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- Simplified Liquid-Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization
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Four self-complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT,dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure.Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high-purity products at each step of the process.The decamers were elaborated from the 3'-end, starting with a 3'-O-benzoylated monomer according to the scheme: monomer + monomer -> dimer + dimer -> tetramer + dimer -> hexamer + tetramer -> decamer.Putity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300-MHz 1H-NMR. spectroscopy.The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1H-NMR. spectra.
- Denny, William A.,Leupin, Werner,Kearns, David R.
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p. 2372 - 2393
(2007/10/02)
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