- SURFACE-MODIFIED CELLS, METHODS OF MAKING AND USING
-
Surface-modified cell containing a cell and a conformal coating on the extracellular surface of the cell are described. The conformal coating contains two or more layers containing particles (e.g. nanoparticles) or macromolecules. The cell is an islet cell, a B cell, or a T cell. The macromolecules or particles are formed from zwitterionic polymers. Covalent linkages are employed to link the particles or macromolecules to a cell surface molecule containing an abiotic functional group, or between macromolecules and/or particles in adjacent layers. Also described are methods of making and using a surface-modified cell.
- -
-
Paragraph 0168
(2021/11/20)
-
- Synthesis method of betaine type zwitterion
-
The invention provides a synthesis method of a betaine type zwitterion, belonging to the technical field of compound synthesis. The synthesis method comprises the following steps: A, adding dimethylaminoethyl methacrylate or dimethylaminopropyl acrylamide into a reactor, adding acetic acid, carrying out cooling in an ice bath, and dropwise adding acrylic acid for a reaction; B, under a nitrogen condition, adding ethylene glycol monomethyl ether, pyridine and dichloromethane into a container, and dropwise adding methanesulfonyl chloride under an ice bath condition for a reaction; C, under the condition of nitrogen, adding sodium into dichloromethane, carrying out heating until sodium is molten, conducting stirring to room temperature, adding 2-dimethylaminoethanol under the ice bath condition, conducting reacting at room temperature, dropwise adding faint yellow transparent liquid under the ice bath condition, and conducting reacting at room temperature to prepare colorless liquid; D, mixing the colorless liquid with acetone to obtain a mixture; and E, under a stirring condition, dropwise adding the colorless liquid into clarified viscous liquid, carrying out stirring at normal temperature, dropwise adding the mixture, and successively carrying out stirring, filtering, washing and drying to obtain the betaine type zwitterion. According to the method, reaction conditions are mildand stable, and product purity is high.
- -
-
Paragraph 0027-0047
(2020/11/23)
-
- Effect of Association on Nucleophilic Addition of (Meth)acrylic Aminoamides to Acrylic Acids in Aqueous Solutions
-
Reactions of nucleophilic addition of N-(3-dimethylaminoalkyl)(meth)acrylamides to acrylic, N-acryloylaminoacetic, and 2-acrylamido-2-methylpropanesulfonic acid with the formation of betaines revealed an unusual dependence of the initial rate and equilibrium conversion on concentration of the reagents. The observed effects were explained by structural features of the reagents and the products.
- Kazantsev,Baruta,Kamorin,Shirshin,Sivokhin
-
p. 641 - 645
(2018/06/14)
-
- Superhydrophilicity and spontaneous spreading on zwitterionic surfaces: Carboxybetaine and sulfobetaine
-
Zwitterionic surfaces are fabricated by grafting sulfobetaine silane (SBSi) and carboxybetaine silane (CBSi) on glass slides. Their wetting behaviors are investigated using water, polar organic liquids, and hexadecane as test liquids. For the CBSi surface, partial wetting is observed, and contact angles of water and hexadecane are lower than 10°, revealing super-amphiphilicity. For the SBSi surface, all test liquids spread spontaneously and contact angles are absent, corresponding to total wetting. The time evolution of the wetting area of a liquid drop can be divided into three types: spread-withdrawal for water, spread-pin for polar organic liquids, and continuous spread for hexadecane. The spontaneous spreading on SBSi surfaces is driven by the high solid-gas interfacial tension and can be characterized by the power law. Although zwitterionic surfaces like both water and hexadecane in ambient air, their preference for water over hexadecane is typically demonstrated by a hexadecane drop in a water environment. Nonetheless, the contact angle of the hexadecane drop is 120°on the CBSi surface, but becomes 180°on the SBSi surface. As the zwitterionic surfaces are immersed in all test liquids, bubbles generally adhere to the CBSi surface but freely move beneath the SBSi surface. Our experimental results clearly show the wettability difference between the CBSi and SBSi surfaces. The former is superhydrophilic, while the latter is total wetting.
- Wu, Cyuan-Jhang,Huang, Chun-Jen,Jiang, Shaoyi,Sheng, Yu-Jane,Tsao, Heng-Kwong
-
p. 24827 - 24834
(2016/03/22)
-
- Cells frozen storage protection composition, the use of this composition and method of cell suspension (by machine translation)
-
This application relates to a cell frozen storage protection composition, the use of this composition and method of cell suspension. The cell suspension containing protection composition having the general formula structure R 1-N + (CH 3) 2-(CH 2) n-R 2 amphoteric molecule, wherein the above-mentioned R 1 to carbon number 1 to 10 linear or branched alkyl, which is optionally substituting group substituted, R 2 to is selected from the group consisting of-COO -, -SO 4-, -SO 3-, and In the group consisting of any one of the group with negative charge, the R 3 to is selected from the group consisting of (meth) acryloxy-alkyl, alkyl, and alkenyl group consisting of the group in; the having the formula structure R 1-N + (CH 3) 2-(CH 2) n-R 2 amphoteric molecule is preferably a sugar beet alkali compound. Said cells frozen storage protection composition can be non-toxic and efficiently implement cells cryopreserved, very high survival rate of cells after recovery without gradient cryopreserved; after recovery cells, may be used directly or using the cells slightly after diluting. (by machine translation)
- -
-
Paragraph 0108
(2017/03/17)
-
- PRODUCTION AND PURIFICATION OF CARBOXYLIC BETAINE ZWITTERIONIC MONOMERS
-
A method is provided for the production and purification of carboxylic betaine-based zwitterionic vinyl monomers, such as (meth)acrylic or (meth)acrylamide monomer containing a betaine-type group of the formula: wherein A is an ester, amide, benzyl, pyridinyl, or imidazolyl; R is hydrogen or methyl; and n is an integer from 0 to 10. The method involves the reaction of acrylic acid with aminoalkyl (meth)acrylate or N-amino-alkyl (meth)acrylamide having a basic tertiary nitrogen atom in the presence of a free radical inhibitor, this reaction leads to a betaine:acid complex that can be either separated from the reaction media into pure betaine:acid complex powder or further purified into pure monomer powder by passing the reaction mixture through a base resin followed by precipitation in a nonsolvent or direct precipitation in a nonsolvent containing an organic base.
- -
-
Paragraph 0029
(2014/09/30)
-