- Simplified Liquid-Phase Preparation of Four Decadeoxyribonucleotides and their Preliminary Spectroscopic Characterization
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Four self-complementary decadeoxyribonucleotides, dApTpApTpApTpApTpApT,dApTpApTpCpGpApTpApT, dApTpApTpGpCpApTpApT and dApApApApApTpTpTpTpT, were chemically synthesized by the phosphotriester procedure.Efforts were concentrated on keeping the procedure as simple as possible, concomitant with obtaining high-purity products at each step of the process.The decamers were elaborated from the 3'-end, starting with a 3'-O-benzoylated monomer according to the scheme: monomer + monomer -> dimer + dimer -> tetramer + dimer -> hexamer + tetramer -> decamer.Putity of intermediates and of the fully blocked decamers were monitored by chromatography and by 300-MHz 1H-NMR. spectroscopy.The deblocked decadeoxyribonucleotides were characterized by their UV., CD., and 1H-NMR. spectra.
- Denny, William A.,Leupin, Werner,Kearns, David R.
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p. 2372 - 2393
(2007/10/02)
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- NEW CONDENSING REAGENTS FOR STEREOSPECIFIC SYNTHESIS OF DINUCLEOSIDE MONOPHOSPHATE ARYL ESTERS
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Condensation of 5'-O-dimethoxytritylnucleoside 3'-O-(o-chlorophenyl)phosphates and 3'-O-benzoylnucleosides with a new condensing reagent, 2,4,6-triisopropylbenzenesulfonyl 5-(pyridin-2-yl) tetrazolide gave o-chlorophenyl ester of protected dinucleoside mo
- Ohtsuka, E.,Tozuka, Z.,Ikehara, M.
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p. 4483 - 4486
(2007/10/02)
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