- 1,2-Diamino-4,5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids
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The reactivity of pyruvic acid with 1,2-diaminobenzenes was investigated as part of a survay of fluorogenic reagents for α-keto acids of biological importance.Of eight 1,2-diaminobenzenes tested, 1,2-diamino-4,5-methylenedioxybenzene was found to be the best reagent in terms of sensitivity and reactivity.The reagent reacts with α-keto acids in an acidic solution to produce the corresponding fluorescent derivatives, which fluorescent most intensely in a neutral medium.The derivatives of ten α-keto acids can be separeted within 18 min by reversed-phase high-performance liquid chromatography with isocratic elution.The detection limits for the acids are at femtomole levels for an injection volume of 10 μl.The fluorescent product from pyruvic acid was characterized as 3-methyl-6,7-methylenedioxy-2(1H)-quinoxalinone.The fluorescence properties of the product are also described.
- Nakamura, Masaru,Hara, Shuuji,Yamaguchi, Masatoshi,Takemori, Yasuyo,Ohkura, Yosuke
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p. 687 - 692
(2007/10/02)
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- Imidazole derivatives
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Tricyclic imidazole derivatives of the formula STR1 wherein R1 is 2-pyridyl optionally substituted by lower alkyl or lower alkoxy, n is the integer 0 or 1, R2 is hydrogen or lower alkyl, R3 and R4, independently, are hydrogen or lower alkyl, A is a group of the formula STR2 m is the integer 2 or 3, R5, R6, R7 and R8, independently, are hydrogen or lower alkyl, and R9 is hydrogen and R10 is hydrogen or lower alkyl or R9 and R10 taken together are oxo, provided that at least one of R3 and R4 is lower alkyl when A is a group of the formula and their pharmaceutically acceptable acid addition salts. The compounds of formula I inhibit gastric acid secretion and prevent the formation of gastric ulcers.
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