- Constructing a triangular metallacycle with salen-Al and its application to a catalytic cyanosilylation reaction
-
A triangular metallosalen-based metallacycle was constructed in quantitative yield by the self-assembly of a 180° bis(pyridyl)salen-Al complex and a 60° diplatinum(ii) acceptor in a 1?:?1 stoichiometric ratio. This metallacycle was then successfully used to cyanosilylate a wide range of benzaldehydes with trimethylsilyl cyanide.
- Li, Bo,Li, Yang,Qiu, Huayu,Xu, Jun,Yin, Shouchun,Zhang, Jinjin,Zhang, Pengfei,Zhang, Yueyue
-
supporting information
p. 10399 - 10402
(2021/10/12)
-
- Stabilization of Hydroxynitrile Lyases from Two Variants of Passion Fruit, Passiflora edulis Sims and Passiflora edulis Forma flavicarpa, by C-Terminal Truncation
-
Because the synthesis of chiral compounds generally requires a broad range of substrate specificity and stable enzymes, screening for better enzymes and/or improvement of enzyme properties through molecular approaches is necessary for sustainable industri
- Nuylert, Aem,Motojima, Fumihiro,Khanongnuch, Chartchai,Hongpattarakere, Tipparat,Asano, Yasuhisa
-
p. 181 - 189
(2019/12/12)
-
- Enantioselective cyanosilylation of aldehydes catalyzed by a multistereogenic salen-Mn(III) complex with a rotatable benzylic group as a helping hand
-
A multistereogenic salen-Mn(iii) complex bearing an aromatic pocket and two benzylic groups as helping hands was found to be efficient in the catalysis of asymmetric cyanosilylation. The salen-Mn catalyst partially mimics the functions of biocatalysts by
- Wei, Yun-Long,Huang, Wei-Sheng,Cui, Yu-Ming,Yang, Ke-Fang,Xu, Zheng,Xu, Li-Wen
-
p. 3098 - 3103
(2015/02/02)
-
- Solid phase behavior in the chiral systems of various 2-hydroxy-2-phenylacetic acid (mandelic acid) derivatives
-
The solid phase behavior of a series of monosubstituted F-, Cl-, Br-, I-, and CH3- and two 2,4-halogen-disubstituted 2-hydroxy-2-phenylacetic acid (mandelic acid) derivatives was investigated. The study includes detailed information about melting temperature, melting enthalpy, X-ray diffraction data, as well as selected binary phase diagrams of the respective chiral systems. Aside from the known metastable conglomerate 2-chloromandelic acid, evidence for two more metastable conglomerates was found.
- Von Langermann, Jan,Temmel, Erik,Seidel-Morgenstern, Andreas,Lorenz, Heike
-
p. 721 - 728
(2015/03/30)
-
- Enantioselective cyanosilylation of aldehydes catalyzed by novel camphor derived Schiff bases-titanium(IV) complexes
-
Five tridentate Schiff bases have been prepared from (1R,2S,3R,4S)-3-amino- 1,7,7-trimethylbicyclo[2.2.1]heptan-2-ol and salicylaldehydes. X-ray structure investigation revealed differences in their molecular conformation, and their titanium(IV) complexes
- B?ocka, Ewelina,Bosiak, Mariusz J.,We?niak, Miros?aw,Ludwiczak, Agnieszka,Wojtczak, Andrzej
-
p. 554 - 562
(2014/05/06)
-
- A novel bifunctional Ti(IV) complex catalyzed asymmetric silylcyanation of aldehydes
-
A novel Lewis acid and Lewis base bifunctional Ti(IV) complex was formed in situ upon the treatment of ligand (R)-3,3′-bis(diphenylphosphinoyl)-BINOL with Ti(PrO-i)4, which is proven to be an efficient catalyst in the asymmetric trimethylsilylc
- Tang, Hongying,Zhang, Zhongbiao
-
-
- Enantioselective hydrocyanation of aldehydes catalyzed by [Li{Ru(phgly)2(binap)}]X (X = Cl, Br)
-
Novel bimetallic complexes [Li{Ru[(S)-phgly]2[(S)-binap]}]X (X = Cl, Br) are readily synthesized by mixing Ru[(S)-phgly]2[(S)-binap] and LiX. A single-crystal X-ray analysis reveals the structure. These bimetallic complexes efficient
- Kurono, Nobuhito,Yoshikawa, Tatsuya,Yamasaki, Mikio,Ohkuma, Takeshi
-
supporting information; experimental part
p. 1254 - 1257
(2011/05/02)
-
- Enantioselective silylcyanation of aldehydes catalyzed by new chiral oxovanadium complex
-
Oxovanadium(V) complex 4 was prepared from VOSO4 and tridentate Schiff base ligand, which was prepared from substituted salicylaldehyde and inexpensive (S)-valine. Asymmetric silylcyanation of the aldehydes catalyzed by catalyst 4 afforded cyanohydrins with good enantioselectivities.
- Chu, Chang-Ying,Hsu, Chu-Tin,Lo, Po Hsiang,Uang, Biing-Jiun
-
scheme or table
p. 1981 - 1984
(2012/03/10)
-
- In situ evaluation of lipase performances through dynamic asymmetric cyanohydrin resolution
-
A dynamic resolution process based on multiple reversible cyanohydrin formation coupled to lipase-mediated transesterification is demonstrated. The resulting process resulted in the efficient evaluation of complex lipase performances in asymmetric cyanohydrin acylate synthesis. Dynamic systems were generated and resolved in situ, and the effects of the reaction conditions could be directly monitored for the overall system. By this concept, the enzyme activity, chemo- and stereoselectivity for all involved substrates could be simultaneously evaluated.
- Sakulsombat, Morakot,Vongvilai, Pornrapee,Ramstroem, Olof
-
experimental part
p. 1112 - 1117
(2011/04/16)
-
- Asymmetric synthesis of a new salen type-titanium complex as the catalyst for asymmetric trimethylsilylcyanation of aldehydes
-
This work describes the asymmetric synthesis of a new salen-type ligand via a Diels-Alder reaction and Curtius rearrangement. The ligand with a norbornane skeleton was used in the trimethylsilylcyanation of aldehydes, but the enantioselectivity was 55%ee. The norborane skeleton was cleaved to destroy this rigidity, and the eanatioselectivity was thereby increased to 85%ee.
- Lin, Zheng-Chang,Chen, Chinpiao
-
experimental part
p. 726 - 737
(2011/04/23)
-
- [Ru(phgly)2(binap)]/Li2CO3: A Highly Active, Robust, and Enantioselective Catalyst for the Cyanosilylation of Aldehydes
-
The right combination: A series of aromatic, heteroaromatic, aliphatic, and α,β-unsaturated aldehydes can be converted into the desired silylated cyanohydrins by reaction with (CH3)3SiCN and a catalyst system consisting of the combination of a chiral ruthenium complex and Li2CO3 (see scheme). The reaction is highly enantioselective and affords the R products with up to 98% ee within 24 h at a substrate-tocatalyst ratio of 10000:1. (Chemical Equation Presented).
- Kurono, Nobuhito,Arai, Kenta,Uemura, Masato,Ohkuma, Takeshi
-
supporting information; experimental part
p. 6643 - 6646
(2009/03/12)
-
- A novel chiral (salen)AlIII complex catalyzed asymmetric cyanosilylation of aldehydes
-
A novel chiral (salen)AlIII complex was synthesized through the reaction of Et2AlCl and salen (R,R)-1 derived from (R,R)-11,12-diamino-9,10-dihydro-9,10-ethanoanthracene. This complex is an efficient catalyst for the asymmetric trimethylsilylcyanation of aldehydes in the presence of tributylphosphane oxide as an additive. The use of 1 mol-% of the complex led to the corresponding cyanohydrins in high yields (85-94%) with good-to-excellent enantioselectivities (42-92%ee). Wiley-VCH Verlag GmbH & Co. KGaA, 2008.
- Zeng, Zebing,Zhao, Guofeng,Zhou, Zhenghong,Tang, Chuchi
-
experimental part
p. 1615 - 1618
(2009/04/11)
-
- Asymmetric addition of trimethylsilyl cyanide to aldehydes promoted by chiral polymeric vanadium(V) salen complex as an efficient and recyclable catalyst
-
The asymmetric addition of trimethylsilyl cyanide to various aldehydes catalyzed by efficient new vanadyl polymeric salen complexes having 12 repeating salen units was investigated at room temperature. An excellent yield of the trimethylsilylether of cyanohydrins (up to 98%) with high chiral induction (96%) in case of 2-methylbenzaldehyde was achieved in 18 h. The catalyst recovered four times with retention of its performance.
- Khan, Noor-ul H.,Agrawal, Santosh,Kureshy, Rukhsana I.,Abdi, Sayed H.R.,Mayani, Vishal J.,Jasra, Raksh V.
-
p. 2659 - 2666
(2007/10/03)
-
- An asymmetric, chemo-enzymatic synthesis of O-acetylcyanohydrins
-
A one-pot chemo-enzymatic synthesis of highly enantiomerically enriched O-acetylcyanohydrins has been developed. The bimetallic (salen)titanium complex 1 is used to convert aldehydes into nonracemic (R)-O-acetylcyanohydrins with 61 to 93 % enantiomeric ex
- Belokon, Yuri N.,Blacker, A. John,Clutterbuck, Lisa A.,Hogg, David,North, Michael,Reeve, Christopher
-
p. 4609 - 4617
(2007/10/03)
-
- A new (R)-hydroxynitrile lyase from Prunus mume: Asymmetric synthesis of cyanohydrins
-
A new hydroxynitrile lyase (HNL) was isolated from the seed of Japanese apricot (Prunus mume). The enzyme has similar properties with HNL isolated from other Prunus species and is FAD containing enzyme. It accepts a large number of unnatural substrates (benzaldehyde and its variant) for the addition of HCN to produce the corresponding cyanohydrins in excellent optical and chemical yields. A new HPLC based enantioselective assay technique was developed for the enzyme, which promotes the addition of KCN to benzaldehyde in a buffered solution (pH=4.5).
- Nanda, Samik,Kato, Yasuo,Asano, Yasuhisa
-
p. 10908 - 10916
(2007/10/03)
-
- A C2-symmetric chiral bipyridyldiol-titanium complex as a catalyst for the asymmetric trimethylsilylcyanation of substituted benzaldehydes
-
This article describes a novel C2-symmetric ligand that comprises a central bipyridine-pinene-derived core and two functionalized two diphenylmethanol subunits. The [8′-(hydroxy-diphenyl-methyl)-10,10, 10′,10′-tetramethyl-[5,5′]bi[6-aza-tricyclo[7.1.1.0 2,7]undecyl]-2(7),3,5,2′(7′),3′, 5′-hexane-8-yl]-diphenyl-methanol 1 is an effective catalyst in the enantioselective addition of trimethylsilylcyanide to various aromatic aldehydes with asymmetric inductions of up to 98% ee. Importantly, the correlation between Hammett substituent constants and the enantiomeric excess, and the electron-releasing group at the meta- and para-positions of substituted benzaldehydes were demonstrated to be associated with the high enantioselectivity of the trimethylsilylcyanation reaction that involves trimethylsilylcyanide.
- Lee, Pei-Ting,Chen, Chinpiao
-
p. 2704 - 2710
(2007/10/03)
-
- Synthesis of a new chiral cyclic o-hydroxynaphthylphosphonodiamide and its application as ligand catalyst in asymmetric silylcyanation of aromatic aldehydes
-
A new chiral cyclic o-hydroxynaphthylphosphonodiamide (+)-2 was synthesized starting from (+)-cis-1,2,2-trimethylcyclopentane-1,3-diamine. The absolute configuration of phosphorus atom was determined as S by X-ray diffraction analysis. Excellent enantiose
- He, Ke,Zhou, Zhenghong,Wang, Lixin,Li, Kangying,Zhao, Guofeng,Zhou, Qilin,Tang, Chuchi
-
p. 1521 - 1524
(2007/10/03)
-
- Highly enantioselective cyanosilylation of aldehydes catalyzed by a chiral oxazaborolidinium ion
-
The chiral oxazaborolidinium salts 1 and 2 are excellent catalysts for the enantioselective cyanosilylation of a wide variety of aldehydes (see Table 1) using trimethylsilyl (TMS) cyanide and triphenylphosphine oxide as the source of a new reactive cyanide donor. This donor appears to be the isocyanophosphorane Ph3P(OTMS)(N=C:) (4). The novel process described herein has several advantages: predictability of absolute configuration of cyanohydrin products from a mechanistic model (3), high yields and very good enantiomeric purity of products (≥90%), and, finally, easy and efficient recovery of the catalytic ligand. Copyright
- Ryu, Do Hyun,Corey
-
p. 8106 - 8107
(2007/10/03)
-
- Development of β-hydroxyamide/titanium complexes for catalytic enantioselective silylcyanation of aldehydes
-
A highly enantioselective addition of trimethylsilylcyanide to aldehydes catalyzed by chiral titanium complexes is described. The chiral titanium complexes were prepared in situ from Ti(OiPr)4 and β-hydroxyamide ligands, that could easily be synthesized from ketopinic acid and C2 symmetrical chiral diamines in a small number of steps. Graphical Abstract.
- Uang, Biing-Jiun,Fu, I-Pin,Hwang, Chyuan-Der,Chang, Chun-Wei,Yang, Chun-Tzu,Hwang, Der-Ren
-
p. 10479 - 10486
(2007/10/03)
-
- Synthesis of some new chiral bifunctional o-hydroxyarylphosphonodiamides and their application as ligands in Ti(IV) complex catalyzed asymmetric silylcyanation of aromatic aldehydes
-
Some new chiral bifunctional o-hydroxyarylphosphonodiamides were synthesized starting from (+)-cis-1,2,2-trimethylcyclopentane-1,3-diamine and the absolute configuration of the phosphorus atom was determined by X-ray diffraction analysis. Excellent enantioselectivity (up to 98% ee) was achieved in asymmetric silylcyanation of aromatic aldehydes using a chiral titanium complex formed in situ from Ti(OiPr)4 and o-hydroxyarylphosphonodiamide as the catalyst. Graphical Abstract.
- He, Ke,Zhou, Zhenghong,Wang, Lixin,Li, Kangying,Zhao, Guofeng,Zhou, Qilin,Tang, Chuchi
-
p. 10505 - 10513
(2007/10/03)
-
- Nitrilases
-
The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
- -
-
-
- Nitrilases, nucleic acids encoding them and methods for making and using them
-
The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
- -
-
-
- Synthesis of some new chiral cyclic o-hydroxylarylphosphonodiamides as ligands for asymmetric silylcyanation of aromatic aldehydes
-
Some new chiral cyclic o-hydroxylarylphosphonodiamides were synthesized from (-)-α-phenylethylamine and found to be efficient ligands for the Ti(OPr-i)4 catalyzed asymmetric trimethylsilylcyanation of aromatic aldehydes. Corresponding cyanohydr
- Yang, Zhuohong,Zhou, Zhenghong,He, Ke,Wang, Lixin,Zhao, Guofeng,Zhou, Qilin,Tang, Chuchi
-
p. 3937 - 3941
(2007/10/03)
-
- Nitrilases
-
The invention relates to nitrilases and to nucleic acids encoding the nitrilases. In addition methods of designing new nitrilases and method of use thereof are also provided. The nitrilases have increased activity and stability at increased pH and temperature.
- -
-
-
- Lipase-catalyzed enantioselective transesterification toward esters of 2-bromo-tolylacetic acids
-
Lipases from Candida antarctica, Pseudomonas cepacia and Rhizomucor miehei were tested in the resolution of seven racemic substrates belonging to the (RS)-2-bromo tolyl acetate ester category, but differing either in the position of the methyl substituent on the acyl part of the aromatic ring, or in the structure of the alkyl group. Lipase-catalyzed kinetic resolution via transesterification reaction between the ester and octanol in octane revealed that, of the three enzymes tested, P. cepacia lipase is the most efficient for resolution of the various racemates, with R-enantiopreference. In addition, the position of the methyl substituent was found to play a key role in governing the enantioselectivity of the reaction. Using P. cepacia lipase and 2-bromo-m/p-tolyl- or 2-bromophenylacetic acid esters E-values of 6.
- Guieysse, David,Salagnad, Christophe,Monsan, Pierre,Remaud-Simeon, Magali
-
p. 317 - 323
(2007/10/03)
-
- An enzyme library approach to biocatalysis: Development of nitrilases for enantioselective production of carboxylic acid derivatives
-
The discovery, from Nature, of a large and diverse set of nitrilases is reported. The utility of this nitrilase library for identifying enzymes that catalyze efficient production of valuable hydroxy carboxylic acid derivatives is demonstrated. Unprecedented enantioselectivity and substrate scope are highlighted for three newly discovered and distinct nitrilases. For example, a wide array of (R)-mandelic acid derivatives and analogues were produced with high rates, yields, and enantiomeric excesses (95-99% ee). We also have found nitrilases that provide direct access to (S)-phenyllactic acid and other aryllactic acid derivatives, again with high yields and enantioselectivities. Finally, different nitrilases have been discovered that catalyze enantiotopic hydrolysis of 3-hydroxyglutaronitrile to afford either enantiomer of 4-cyano-3-hydroxybutyric acid with high enantiomeric excesses (>95% ee). The first enzymes are reported that effect this transformation to furnish the (R)-4-cyano-3-hydroxybutyric acid which is a precursor to the blockbuster drug Lipitor. Copyright
- DeSantis, Grace,Zhu, Zuolin,Greenberg, William A.,Wong, Kelvin,Chaplin, Jenny,Hanson, Sarah R.,Farwell, Bob,Nicholson, Lawrence W.,Rand, Cynthia L.,Weiner, David P.,Robertson, Dan E.,Burk, Mark J.
-
p. 9024 - 9025
(2007/10/03)
-
- Synthesis of a new chiral o-hydroxyaryldiazaphosphonodiamide as ligand catalysts for asymmetric silylcyanation of aromatic aldehydes
-
A new chiral o-hydroxyaryldiazaphosphonodiamide 1 was synthesized from (-)-α-methylbenzylamine, it was found to be efficient ligand for the Ti(O-iPr)4 catalyzed asymmetric trimethylsilylcyanation of aldehydes. Corresponding aromatic cyanohydrin
- Yang, Zhuo-Hong,Zhou, Zheng-Hong,Wang, Li-Xin,Li, Kang-Ying,Zhou, Qi-Lin,Tang, Chu-Chi
-
p. 2751 - 2756
(2007/10/03)
-
- 1,2-Diphenylethylenediamine linked chiral Ti(IV) complex - A new entry to the highly enantioselective silylcyanation of aliphatic and aromatic aldehydes
-
Highly enantioselective silylcyanation of aliphatic and aromatic aldehydes was achieved by using a 1,2-diphenylethylenediamine linked chiral Ti(IV) complex as the catalyst.
- Chang, Chun-Wei,Yang, Chun-Tzu,Hwang, Chyuan-Der,Uang, Biing-Jiun
-
-
- Titanium and ruthenium binaphthyl Schiff base complexes as catalysts for asymmetric trimethylsilylcyanation of aldehydes
-
Investigations on the catalytic behaviour of titanium complexes formed in situ from Ti(OPri)4 and a variety of Schiff bases, mainly the binaphthyl derivatives 2,2′-bis(3-R1-5-R 2-2-hydroxybenzylideneamino)-1,1′-binaphthyl, toward the asymmetric trimethylsilylcyanation of some aromatic and aliphatic aldehydes demonstrated that the titanium complex of the binaphthyl Schiff base with R1 = R2 = But is one of the best catalysts for such a process, with an enantiomeric excess (e.e.) as high as 96% obtained for m-tolualdehyde. Crystal structure determination of a nitrosylruthenium complex, [RuII(L)(NO)Cl] (L is the dianion of the binaphthyl Schiff base with R1 = R2 = Cl), revealed that the complex assumes a cis-β configuration with the binaphthyl moiety having a dihedral angle of 70.2°. After treatment with AgPF6, the ruthenium complex also exhibited a good catalytic property for the trimethylsilylcyanation of benzaldehyde albeit with a lower e.e. (24%). The Royal Society of Chemistry 1999.
- Zhou, Xiang-Ge,Huang, Jie-Sheng,Ko, Po-Hung,Cheung, Kung-Kai,Che, Chi-Ming
-
p. 3303 - 3309
(2007/10/03)
-
- Asymmetric addition of trimethylsilyl cyanide to aldehydes catalysed by chiral (salen)TiIV complexes
-
The asymmetric addition of trimethylsilyl cyanide to a range of aldehydes is catalysed by the titanium(IV) complexes of chiral salen derivatives 3-6. The enantiomeric excess of the resulting cyanohydrins is predominantly dependent upon the structure of the aldehyde, with electron-rich aromatic aldehydes giving best results. Electron-deficient aromatic aldehydes give cyanohydrins with low enantiomeric excesses, whilst ketones fail to react.
- Belokon', Yuri,Flego, Massimilano,Ikonnikov, Nicolai,Moscalenko, Margarita,North, Michael,Orizu, Charles,Tararov, Vitali,Tasinazzo, Michela
-
p. 1293 - 1295
(2007/10/03)
-
- Reductive Coupling of Benzoyl Cyanide and Carbonyl Compounds by Aqueous Ti(III) Ions. A New Convenient and Selective Access to the Less Stable Mixed Benzoins
-
The reactive species formed by the Ti(III) ion reduction of benzoyl cyanide (1) adds to the C-atom of carbonyl compounds 2 under simple experimental conditions.The intermediate 1,2-diols 3 are smoothly converted, without isolation, into the less thermodynamically stable mixed benzoins 4, which are not accessible by the classical benzoin condensation.The possible mechanisms involved in the reaction are discussed.
- Clerici, Angelo,Porta, Ombretta
-
p. 2889 - 2893
(2007/10/02)
-
- A catalyst for asymmetric induction
-
A catalyst for asymmetric induction is provided which comprises a titanium (IV) alkoxide and an amino acid amide derivative of formula (I): wherein R1 is isopropyl, isobutyl, sec-butyl, tert-butyl, phenyl or benzyl, R2 and R3 are the same or different and each is lower alkyl, C3 to C8 cycloalkyl, unsubstituted or substituted phenyl or hydrogen or R2 and R3 together form a divalent C3 to C7 alkylene group optionally containing one or more hetero atoms, R4 is chlorine, bromine, iodine, lower alkyl or unsubstituted or substituted phenyl, R5, R6 and R7 are the same or different and each is hydrogen, halogen, methyl or methoxy or R5 is as defined above and R6 and R7 together form -CH=CH-CH=CH- or -OCH2O- or R7 is as defined above and R5 and R6 together form -CH=CH-CH=CH- or -OCH2O- and * denotes an absolute configuration of S or R.
- -
-
-
- Peptide-titanium complex as catalyst for asymmetric addition of hydrogen cyanide to aldehyde
-
The complex of titanium ethoxide and an acyclic dipeptide ester whose terminal amino group is modified to a salicylal-type Schiff base catalyzes the asymmetric addition of hydrogen cyanide to aldehydes with high enantioselectivity. In the reaction of benzaldehyde and hydrogen cyanide, (R)-mandelonitrile is obtained with an enantiomeric excess of 90% when N-((2-hydroxy-1-naphthyl)methylene)-(S)-valyl-(S)-tryptophan methyl ester is employed. In place of the dipeptide, the amide derivatives of an amino acid modified by substituted salicylaldehyde, such as N-(3,5-dibromosalicylidene)-(S)-valine piperidide, exhibit an entirely opposite stereoselectivity to yield S-cyanohydrins with optical purities up to 97% ee. This novel peptide-titanium complex, therefore, enables us to afford optically active cyanohydrins of both absolute configurations by using natural S-amino acids as chiral auxiliaries.
- Nitta, Hideaki,Yu, Donghai,Kudo, Masanobu,Mori, Atsunori,Inoue, Shohei
-
p. 7969 - 7975
(2007/10/02)
-
- Hydroxynitrile lyases from almond and sorghum as biocatalysts
-
Hydroxynitrile lyases from sweet almond (E.C.4.1.2.10) and from sorghum biocolor (E.C.4.1.2.11) have been purified to homogeneity by ion-exchange chromatography.These enzymes catalyse the decomposition of α-hydroxynitriles to aldehydes and hydrocyanic acid (HCN) and show great promise for synthetic applications in the reverse reaction: the stereospecific addition of HCN to aldehydes to form enantio-pure α-hydroxynitriles.Physical and kinetic characteristics of the two dominating isoenzymes in each source were compared and revaled appreciable species-specific differences in substrate specificity between iso forms within one species, were, however, found to be small.The native molecular weight for the sorghum lyase was found to be 95kDa.This is in contrast with an earlier reported value of 180 kDa.From the kinetic parameters Km and Vmax, a specificity constant was calculated, which can be used to predict the potential application of oxynitrilase as a biocatalyst for specific synthetic purposes.
- Smitskamp-Wilms, E.,Brusse, J.,Gen, A. van der,Scharrenburg, G.J.M. van,Sloothaak, J. B.
-
p. 209 - 215
(2007/10/02)
-
- Enzymatic Preparation of Optically Active Cyanohydrin Acetates
-
A series of cyanohydrin acetates (1)-(47) of widely varying structures, potential chiral building blocks for numerous synthetic applications, has been prepared in good chemical and often high optical yields by enzymatic hydrolysis of their racemic acetates in the presence of an ester hydrolase from Pseudomonas sp.
- Almsick, Andreas van,Buddrus, Joachim,Hoenicke-Schmidt, Petra,Laumen, Kurt,Schneider, Manfred P.
-
p. 1391 - 1393
(2007/10/02)
-
- Asymmetric Addition of Hydrogen Cyanide to Substituted Benzaldehydes Catalyzed by a Synthetic Cyclic Peptide, Cyclo((S)-phenylalanyl-(S)-histidyl)
-
Using cyclo((S)-phenylalanyl-(S)-histidyl) as catalyst, optically active cyanohydrins from substituted benzaldehydes with p-methyl, m-methyl, o-methyl, m-methoxyl, or m-phenoxyl group were obtained with optical yield of 82-33percent.For the asymmetric cyanohydrin synthesis, nonpolar solvent such as benzene or carbon tetrachloride was advantageous, while on asymmetric synthesis took place in methanol or dimethyl sulfoxide.
- Kobayashi, Yoshiyuki,Asada, Shoichi,Watanabe, Ichigen,Hayashi, Hiroaki,Motoo, Yoshiyuki,Inoue, Shohei
-
p. 893 - 896
(2007/10/02)
-