- Sequence-specific cleavage of RNA using macrocyclic lanthanide complexes conjugated to oligonucleotides: A structure activity study
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A series of lanthanide complexes were synthesized and conjugated to an oligodeoxynucleotide. Sequence-specific cleavage of a complementary RNA by the conjugates was performed and results are discussed in terms of structure of the complexes.
- Hall, Jonathan,Huesken, Dieter,Haener, Robert
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p. 1357 - 1368
(2007/10/03)
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- Purines, Pyrimidines, and Imidazoles. Part 58. Synthesis and Reactions of some Imidazole-2,4-dicarboxylic Acid Derivatives
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Diethyl 5-amino-1-(p-methoxybenzyl)imidazole-2,4-dicarboxylate (3b) has been prepared from ethyl 2-amino-2-cyanoacetate and ethyl 1-ethoxycarbonylformimidate hydrochloride or ethyl triethoxyacetate followed by reaction with p-methoxybenzylamine.The diester, with one equivalent of dilute sodium hydroxide solution, furnished ethyl 5-amino-1-(p-methoxybenzyl)imidazole-4-carboxylate (7a).Diazotisation of the diester (3b) and reaction of the diazonium salt with copper(I) chloride produced diethyl 5-chloro-1-(p-methoxybenzyl)imidazole-2,4-dicarboxylate (9a) which, with dilute alkali, gave ethyl 5-chloro-1-(p-methoxybenzyl)imidazole-4-carboxylate (7b).Diazotisation of the diester (3b) in the presence of hypophosphorous acid gave diethyl-1-(p-methoxybenzyl)imidazole-2,4-carboxylate (9b) which, when hydrogenated over palladium-charcoal, produced diethyl imidazole-2,4-dicarboxylate (9c).This with bromine (chlorine) gave diethyl 5-bromo(chloro)imidazole-2,4-dicarboxylate (9d and e).The chloro derivative (9a) was similarly hydrogenated using palladium-charcoal to produce diethyl 5-chloroimidazole-2,4-dicarboxylate (9e).
- Brown, Tom,Shaw, Gordon,Durant, Graham J.
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p. 809 - 812
(2007/10/02)
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