- Structure-Guided Modification of Heterocyclic Antagonists of the P2Y14 Receptor
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The P2Y14 receptor (P2Y14R) mediates inflammatory activity by activating neutrophil motility, but few classes of antagonists are known. We have explored the structure-activity relationship of a 3-(4-phenyl-1H-1,2,3-triazol-1-yl)-5-(aryl)benzoic acid antagonist scaffold, assisted by docking and molecular dynamics (MD) simulation at a P2Y14R homology model. A computational pipeline using the High Throughput MD Python environment guided the analogue design. Selection of candidates was based upon ligand-protein shape and complementarity and the persistence of ligand-protein interactions over time. Predictions of a favorable substitution of a 5-phenyl group with thiophene and an insertion of a three-methylene spacer between the 5-aromatic and alkyl amino moieties were largely consistent with empirical results. The substitution of a key carboxylate group on the core phenyl ring with tetrazole or truncation of the 5-aryl group reduced affinity. The most potent antagonists, using a fluorescent assay, were a primary 3-aminopropyl congener 20 (MRS4458) and phenyl p-carboxamide 30 (MRS4478).
- Yu, Jinha,Ciancetta, Antonella,Dudas, Steven,Duca, Sierra,Lottermoser, Justine,Jacobson, Kenneth A.
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supporting information
p. 4860 - 4882
(2018/06/20)
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- Design, synthesis and biological evaluation of indole derivatives as Vif inhibitors
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The crystal structure of viral infectivity factor (Vif) was reported recently, which makes it possible to design new inhibitors against Vif by structure-based drug design. Through analysis of the protein surface of Vif, the C2 pocket located in the N-terminal was found, which is suit for developing small molecular inhibitors. Then, in our article, fragment-based virtual screening (FBVS) was conducted and a series of fragments was obtained, among which, Zif-1 bearing indole scaffold and pyridine ring can form H-bonds with Tyr148 and Ile155. Subsequently, 19 derivatives of Zif-1 were synthesized. Through the immune-fluorescence staining and Western blot assays, Zif-15 shows potent activity in inhibiting Vif-mediated A3G degradation. Further docking experiment shows that Zif-15 form H-bond interactions with residues His139, Tyr148 and Ile155. Therefore, Zif-15 is a promising lead compound against Vif that can be used to treat AIDS.
- Pu, Chunlan,Luo, Rong-Hua,Zhang, Mengqi,Hou, Xueyan,Yan, Guoyi,Luo, Jiang,Zheng, Yong-Tang,Li, Rui
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supporting information
p. 4150 - 4155
(2017/08/22)
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- A method of preparing amide compounds
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The invention belongs to the field of organic compound preparation, and particularly relates to a method for preparing an amide compound. Specifically, cyano compounds are subjected to selective hydrolysis with a mixed solvent of a protic solvent and a non protonic solvent as a reaction medium under the conditions of heating and alkaline catalysis in the air atmosphere, so as to efficiently produce corresponding amide compounds; at the same time, carbon-carbon coupling reaction can be further utilized to react a compound containing cyano group and halogen group with a boronic acid compound, so as to prepare coupled amide compound through a one-step preparation method. The method avoids the usage of traditional toxic and harmful oxidants such as hydrogen peroxide, and is simple, efficient and green synthesis method for preparing the amide compound.
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Paragraph 0033-0036
(2017/02/09)
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- TETRAHYDRONAPHTHYRIDINE DERIVATIVE
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The invention relates to 6-[2-(1-Isopropyl-piperidin-4-yloxy)-7,8-dihydro-5H-[1 ,6]naphthyridin-6-yl]-nicotinamide of Formula (I) and to processes for the preparation of, compositions containing and the uses of, the compound. The compound is an H3 ligand and is useful in numerous diseases, disorders and conditions, in particular inflammatory, allergic and respiratory diseases, disorders and conditions.
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Page/Page column 18-19
(2010/11/27)
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