- A Multi-responsive Fluorescent Probe Reveals Mitochondrial Nucleoprotein Dynamics with Reactive Oxygen Species Regulation through Super-resolution Imaging
-
Understanding the biomolecular interactions in a specific organelle has been a long-standing challenge because it requires super-resolution imaging to resolve the spatial locations and dynamic interactions of multiple biomacromolecules. Two key difficulties are the scarcity of suitable probes for super-resolution nanoscopy and the complications that arise from the use of multiple probes. Herein, we report a quinolinium derivative probe that is selectively enriched in mitochondria and switches on in three different fluorescence modes in response to hydrogen peroxide (H2O2), proteins, and nucleic acids, enabling the visualization of mitochondrial nucleoprotein dynamics. STED nanoscopy reveals that the proteins localize at mitochondrial cristae and largely fuse with nucleic acids to form nucleoproteins, whereas increasing H2O2 level leads to disassociation of nucleic acid–protein complexes.
- Chen, Juan,Fu, Yao,Han, Guangmei,Han, Xinya,Hu, Zhangjun,Liu, Bianhua,Liu, Zhengjie,Tian, Xiaohe,Yang, Guanqing,Zhang, Ruilong,Zhang, Zhongping
-
-
Read Online
- Monomolecular fluorescent probe for simultaneous super-resolution imaging of mitochondrial hydrogen peroxide, proteins and nucleic acids as well as preparation and application of monomolecular fluorescent probe
-
The invention discloses a monomolecular fluorescent probe for simultaneous super-resolution imaging of mitochondrial hydrogen peroxide, proteins and nucleic acids as well as preparation and application of the monomolecular fluorescent probe. The monomolecular fluorescent probe is a cationic quinoline-vinyl-N, N-dimethylaniline borate derivative, can selectively enter cell mitochondria and interactwith hydrogen peroxide, protein hydrophobic regions and nucleic acids to generate a distinguishable fluorescence spectrum for three-channel imaging. The three fluorescence emission wavelengths are relatively long and are suitable for super-resolution imaging analysis of spatial distribution of hydrogen peroxide, proteins and nucleic acids in mitochondria and intermolecular interaction. The monomolecular three-channel fluorescence imaging fluorescence probe provided by the invention can solve the problems of severe cytotoxicity caused by simultaneous use of a plurality of fluorescence probes for imaging, complication of biological distribution and spectral differentiation of fluorescence probes in cells and the like.
- -
-
Paragraph 0028
(2020/04/01)
-
