Semisynthesis of a Bacterium with Non-canonical Cell-Wall Cross-Links
The cell wall is an elaborate framework of peptidoglycan that serves to protect the bacterium against osmotic challenge. This exoskeleton is composed of repeating saccharides covalently cross-linked by peptide stems. The general structure of the cell wall is widely conserved across diverse Gram-negative bacteria. To begin to explore the biological consequence of introducing non-canonical cross-links into the cell wall of Escherichia coli, we generated a bacterium where up to 31percent of the cell-wall cross-links are formed by a non-enzymatic reaction between a sulfonyl fluoride and an amino group. Bacteria with these non-canonical cell-wall cross-links achieve a high optical density in culture, divide and elongate successfully, and display no loss of outer membrane integrity. This work represents a first step in the design of bacteria with non-canonical "synthetic"cell walls.
Chen, Jason S.,Dik, David A.,Schultz, Peter G.,Webb, Bill,Zhang, Nan
supporting information
p. 10910 - 10913
(2020/07/08)
Synthesis of [L-m-Tyr1]- and [D-m-Tyr1]-leucine-enkephalin via the REMA method
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Van den Braken-Van Leersum,Maat
p. 110 - 111
(2007/10/02)
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