- Impact of the surface charge of polydiacetylene micelles on their interaction with human innate immune protein C1q and the complement system
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Polydiacetylene (pDA) micelles have been demonstrated to be effective drug carriers for cancer therapy in mouse model. However, little is known about their interaction with the human complement system, which constitutes an important part of the innate immune system and can cause severe hypersensitivity reactions. Herein, we investigate the influence of micelle surface charge on the binding of complement protein C1q, the target recognition unit that activates the classical complement pathway and performs a range of other important physiological functions. Besides the classical pathway, we also investigate the surface charge effect on complement activities through the other activation pathways, namely, the MBL-dependent lectin pathway and the alternative pathway. We synthesized three samples of pDA micelles bearing neutral, anionic, and cationic surface charge motifs, respectively. Surface plasmon resonance showed that none of these micelles interacted with C1q. Results from serum complement activation assays indicated that all micelles were inert to complement, except for the anionic pDA micelles, which activated the alternative pathway.
- Thielens, Nicole M.,Belime, Agathe,Gravel, Edmond,Ancelet, Sarah,Caneiro, Charlotte,Doris, Eric,Ling, Wai Li
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- Aqueous 1,3-dipolar cycloadditions promoted by copper nanoparticles in polydiacetylene micelles
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A novel colloidal catalyst was developed through the encapsulation of cuprous oxide nanoparticles in polydiacetylene micelles. The Cu-based catalyst was applied to the Huisgen cycloaddition reaction in fully aqueous medium. The process neither requires he
- Clarisse, Damien,Prakash, Praveen,Geertsen, Valérie,Miserque, Frédéric,Gravel, Edmond,Doris, Eric
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supporting information
p. 3112 - 3115
(2017/07/24)
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- Fine-tuning the morphology of self-assembled nanostructures of propargyl ammonium-based amphiphiles
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N-Methyl-N-(pentacosa-10,12-diyn)-propargylamine organizes itself into an unusual supramolecular pH- and thermo-responsive system. Studies have showed that submillimetric length hollow laths form this unique structure in the presence of hydrochloric acid. Specific chemical modifications on the initial molecule and small-angle neutron scattering experiments were performed to understand the structure of this system. Our results allow us to suggest a possible structure of the laths.
- Morin, Emmanuelle,Guenet, Jean-Michel,Díaz, David D.,Remy, Jean-Serge,Wagner, Alain
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experimental part
p. 12495 - 12500
(2011/02/27)
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- The synthesis of conjugated diacetylene monomers for the fabrication of polymerized monolayer assemblies
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Symmetrical disulfides containing conjugated diacetylene groups for use in forming polymerized self-assembled monolayers have been fabricated using common synthetic techniques. In contrast with previous reports, the proposed synthetic route does not require the use of hexamethylphosphoramide (HMPA) or the incorporation of an ester moiety near the surface attachment site. Overall yields of 20-30% are readily achieved for this four-step synthesis.
- Mowery, Mark D.,Evans, Christine E.
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