- Photo-Cross-Linked Polydimethylacrylamide Hydrogels as Porogens for Mesoporous Alumina
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Dimethylacrylamide-based hydrogels were utilized as porogenic matrices in the synthesis of mesoporous aluminum oxide (γ-Al2O3) with specific BET surface areas up to 360 m2 g–1. Polymers with molecular mass in the range 12000–35000 g mol–1 were synthesized from dimethylacrylamide and various comonomers by free-radical polymerization. Photo-cross-linking of the polymers and impregnation with aluminum nitrate [Al(NO3)3] was carried out in a single step, followed by formation of Al(OH)3/AlO(OH) and subsequent calcination. Calcination led to the formation of mesoporous Al2O3 and simultaneous combustion of the hydrogel. The structural properties of the products were characterized by powder XRD, N2 physisorption analysis, Hg intrusion porosimetry, and thermogravimetric analysis.
- Weinberger, Christian,Chen, Zimei,Birnbaum, Wolfgang,Kuckling, Dirk,Tiemann, Michael
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- Facile fabrication method of hydrophobic-associating cross-linking hydrogel with outstanding mechanical performance and self-healing property in the absence of surfactants
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A cationic surfactant monomer, dimethyldodecyl(2-acrylamidoethyl)ammonium bromide (AMQC12) was synthesized. A family of hydrophobic-associating cross-linking hydrogels (HAC-gels) fabricated via the self-assembly of amphiphilic multiblock copolymers of acrylamide and AMQC12 can be synthesized by free-radical aqueous solution micelle copolymerization in the absence of surfactants using the one-pot method. The HAC-gels possessed outstanding mechanical performance, with optimal tensile strength, compressive strength, and elongation at break of 250 kPa, 14 MPa, and 1850%, respectively. Meanwhile, the HAC-gels exhibited self-healing property, and tetrahydrofuran (THF) significantly accelerated their self-healing process. The recovery hysteresis of hydrophobic-associating hydrogels prepared in the presence of surfactants can be eliminated because of homogeneity of the hydrogel network and the dynamic and mobile properties of physical cross-linking junctions. Investigations on the mechanical property and structure evolution of hydrogels revealed that the hydrophobic-associating interaction was the driving force of self-assembly of amphiphilic multiblock copolymers. Furthermore, spherical micelles and macroscopic cross-linking network can be easily switched reversibly by regulating copolymers concentration.
- Xu, Kun,An, Huiyong,Lu, Cuige,Tan, Ying,Li, Pengchong,Wang, Pixin
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- Non-Viral CRISPR/Cas Gene Editing In Vitro and In Vivo Enabled by Synthetic Nanoparticle Co-Delivery of Cas9 mRNA and sgRNA
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CRISPR/Cas is a revolutionary gene editing technology with wide-ranging utility. The safe, non-viral delivery of CRISPR/Cas components would greatly improve future therapeutic utility. We report the synthesis and development of zwitterionic amino lipids (ZALs) that are uniquely able to (co)deliver long RNAs including Cas9 mRNA and sgRNAs. ZAL nanoparticle (ZNP) delivery of low sgRNA doses (15 nm) reduces protein expression by >90 % in cells. In contrast to transient therapies (such as RNAi), we show that ZNP delivery of sgRNA enables permanent DNA editing with an indefinitely sustained 95 % decrease in protein expression. ZNP delivery of mRNA results in high protein expression at low doses in vitro (?1). Intravenous co-delivery of Cas9 mRNA and sgLoxP induced expression of floxed tdTomato in the liver, kidneys, and lungs of engineered mice. ZNPs provide a chemical guide for rational design of long RNA carriers, and represent a promising step towards improving the safety and utility of gene editing.
- Miller, Jason B.,Zhang, Shuyuan,Kos, Petra,Xiong, Hu,Zhou, Kejin,Perelman, Sofya S.,Zhu, Hao,Siegwart, Daniel J.
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supporting information
p. 1059 - 1063
(2017/01/18)
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- CATIONIC SULFONAMIDE AMINO LIPIDS AND AMPHIPHILIC ZWITTERIONIC AMINO LIPIDS
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The present disclosure provides one or more amino lipids such as an amino lipids containing a sulfonic acid or sulfonic acid derivative of the formulas: Formula (I) or (IV) wherein the variables are as defined herein. These amino lipids may be used in compositions with one or more helper lipids and a nucleic acid therapeutic agent. These compositions may be used to treat a disease or disorder such as cancer, cystic fibrosis, or other genetic diseases.
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Page/Page column 53; 109
(2017/12/18)
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- Ink composition for inkjet printing, ink set, and image forming method
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The invention provides an ink composition for inkjet printing, the ink composition containing water, a coloring material, a polymerizable compound, a polymerization initiator represented by the following Formula (X), and polymer particles having a glass transition temperature of 90°C or higher, a mass ratio of the polymer particles to the polymerization initiator represented by Formula (X) being in a range of from 1:10 to 10:1 1 [in Formula (X), each of RX1, RX2, RX3, and RX4 independently represents a hydrogen atom or a substituent; and n represents an integer from 1 to 4].
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- PHOTO-VINYL LINKING AGENTS
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Embodiments of the invention include linking agents including photo groups and vinyl groups and coatings and devices that incorporate such linking agents, along with related methods. Exemplary methods herein include methods of priming substrates and methods of coating substrates using compounds having the formula R1—X—R2, wherein R1 is a radical comprising a vinyl group, X is a radical comprising from about one to about twenty carbon atoms, and R2 is a radical comprising a photoreactive group. Embodiments herein also include linking agents having the formula R1—X—R2, wherein R1 is a radical comprising a vinyl group, X is a radical comprising from about one to about twenty carbon atoms, and R2 is a radical comprising a photoreactive group. Other embodiments are also included herein.
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Page/Page column
(2013/06/26)
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- Micro-carrier cell culture
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Insoluble cationic acrylamide copolymers for use as cell growth carriers and a novel copolymer of dimethylaminopropylmethacrylamide, acrylamide and methylene-bis-acrylamide useful as both a carrier for cell growth and as a medium for ion exchange chromatography of aqueous protein solutions.
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- Process for the manufacture of α,β-unsaturated N-substituted carboxylic acid amides
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Process for the manufacture of α,β-unsaturated N-substituted carboxylic acid amides of the general formula STR1 in which R1 represents H or CH3 R2 represents H or CH3, and Y represents a divalent straight-chain or branched organic radical having 2-30 carbon atoms, and R3 represents H or the radical of an amine of the formula --N(R4)(R5), in which R4 and R5 represent alkyl radicals having 1 to 4 carbon atoms, by reacting β-substituted carboxylic acid amides of the formula STR2 in which Z represents OH or the radical R6 O--, in which R6 is an alkyl radical having 1 to 4 carbon atoms, with amines of the general formula at temperatures in the range of 100° to 200° C., preferably 120° to 175° C., with the elimination of ammonia, and heating the resulting N-substituted β-hydroxycarboxylic or β-alkoxycarboxylic acid amides in the presence of catalysts, water or alcohol, respectively, being split off. The water is split off at temperatures of 100°-250° C. with acidic catalysts such as phosphoric acid, or basic catalysts such as sodium hydroxide, and alcohol is split off at 70°-150° C. with basic catalysts such as sodium or potassium hydroxide. The reaction product is separated by distillation, optionally in vacuo.
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