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the in vitro determination of antimalarial activity a 96-
well plate assay was used.19 To each well, 0.2 mL of a
suspension of P. falciparum infected erythrocytes with
2% haematocrit and 0.4% parasitaemia was added.
Then, a serial dilution of the test compounds was pre-
pared on the plate. After incubation for 48 h, 0.8 lCi
[3H]hypoxanthine in 50 lL medium was added to each
well. The plates were further incubated for 24 h. The
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This study was supported by grants from the European
Commission (QLK2-CT-2002-00887) and INTAS (03-
51-4077). Technical assistance was provided by Dajana
Henschker.
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