ACS Medicinal Chemistry Letters
Letter
four strains, S. aureus ATCC 29213 (E), MRSA2 (G), P.
aeruginosa PA01 (I), and M. smegmatis MC2-155 (J), contained
no genes for which we probed. PCR analysis was previously
performed for strains B and E−G.26 While this data does not
confirm that the proteins associated with these genes are
actively expressed under the conditions tested, it does inform us
that inactivity of compounds on strains that did not have a
positive hit in the probing experiments may have a means of
deactivating AGs other than AMEs.
ACKNOWLEDGMENTS
■
This work was supported by startup funds from the University
of Kentucky (to S.G.-T.) and by the National Institutes of
Health (NIH) grant AI090048 (to S.G.-T.).
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ASSOCIATED CONTENT
■
S
* Supporting Information
The Supporting Information is available free of charge on the
(i) Materials and methods, (ii) all experimental protocols
for the synthesis of compounds and their character-
ization, (iii) experimental protocols for determination of
AME activity by UV−vis assays, MIC values determi-
nation, and identification of AMEs in bacterial strains,
1
and (iv) all H and 13C NMR spectra for the final
AUTHOR INFORMATION
■
Corresponding Author
859-257-7585.
Present Address
∥Memorial Sloan Kettering, New York, New York 10065,
United States.
Author Contributions
§These authors contributed equally to this work. The
manuscript was written through contributions of all authors.
All authors have given approval to the final version of the
manuscript.
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and characterization of inhibitors of the aminoglycoside resistance
acetyltransferase Eis from Mycobacterium tuberculosis. ChemMedChem
2012, 7, 73−77.
(18) Li, Y.; Green, K. D.; Johnson, B. R.; Garneau-Tsodikova, S.
Inhibition of aminoglycoside acetyltransferase resistance enzymes by
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Notes
The authors declare no competing financial interest.
E
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