7268
M. E. Østergaard et al. / Bioorg. Med. Chem. Lett. 20 (2010) 7265–7268
Students, Univ. Idaho (M.E.Ø.) is greatly appreciated. We greatly
appreciate the assistance of Ms. B.M. Dahl (University of Copenha-
gen) during oligonucleotides preparation. We thank Dr. Andrzej J.
Paszczynski and Dr. Lee Deobald from the Murdock Mass Spec-
trometry Center (Environmental Biotechnology Institute, Univ. Ida-
ho) for mass spectrometric analysis.
Supplementary data
Supplementary data (protocols for synthesis, purification and
characterization of ONs, thermal denaturation studies and fluores-
cence experiments; representative thermal denaturation profiles
and additional fluorescence emission spectra) associated with this
Figure 2. Fluorescence intensity of single stranded probes (SSPs) and of the
corresponding duplexes with complementary DNA/RNA. Intensity recorded at
kem = 382 nm at T = 5 °C.
References and notes
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and DNA/RNA targets (Table 1), may reflect the fact that cytosine
moieties are effective quenchers of pyrene fluorescence.9,20 None-
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with DNA/RNA complements (Fig. 2).
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the nucleobase moiety of the Glowing LNA monomer and/or the
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of sequence contexts. These characteristics, in concert with recent
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Glowing LNA composed of different probe backbones (PS-DNA,
RNA O20-Me RNA) and architectures,8b render Glowing LNA as
promising diagnostic probes.
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Acknowledgments
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Financial support from Idaho NSF EPSCoR (P.J.H.), the BANTech
Center at Univ. of Idaho (P.J.H.), The Danish National Research
Foundation (J.W.) and a scholarship from the College of Graduate