10.1002/adsc.202000898
Advanced Synthesis & Catalysis
for X-ray diffraction. The diffraction data of crystals were
collected at beamline BL17U1 of the Shanghai
Synchrotron Radiation Facility (SSRF),[18] and the software
HKL2000 was used for data indexing, integrating and
scaling.[19] The initial structure model of mutants was
obtained by molecular replacement using the VrEH2M263N
(PDB ID: 5Y6Y) as search template. Then the structure
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and refinement result are listed in Table S4. The molecular
docking was carried out using the software
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locations of the catalytic residues. The programs Autogrid 4
and Autodock 4 were performed by default.
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Acknowledgements
[9] M. Kotik, W. Zhao, G. Iacazio, A. Archelas, J. Mol. Catal. B-
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[10] F. L. Li, X. D. Kong, Q. Chen, Y. C. Zheng, Q. Xu, F. F.
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This work was financially supported by The National
Key Research and Development Program of China
(2019YFA09005000), The
National
Natural
Science Foundation of China (21922804 & 21672063 &
21536004 & 21871085), and the Fundamental Research
Funds for the Central Universities (22221818014); We are
very grateful for the access to beamline BL17U1 at
Shanghai Synchrotron Radiation Facility and thank the
beamline staff for technical help. We also thank Simon
Partridge, PhD, for editing the English text of a draft of this
manuscript.
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