Journal of Agricultural and Food Chemistry
ARTICLE
Table 2. Some Kinetic Properties of the Enzymatic Esterification of Ferulic Acid in [Hmim][PF6]/Isooctane and [Omim][PF6]/
Isooctane Systemsa
Vmax (mM h-1 (g enzyme)-1
)
Km (mM)
Ea (kJ mol-1
)
3
[Hmim][PF6]/isooctanea
[Omim][PF6]/isooctanea
2-butanone/isooctaneb
7.75 ( 0.38
4.33 ( 0.21
1.62 ( 0.10
28.06 ( 1.41
23.51 ( 1.18
24.06 ( 0.95
52.72 ( 3.26
66.15 ( 3.64
24.99 ( 1.29
a All kinetic assays were done at 60 °C with an excessive and constant concentration of oleyl alcohol. For both IL-binary systems, the variation of the
concentration of ferulic acid is in the range of 0.01-0.10 mol L-1. b For 2-butanone/isooctane, the variation of the concentration of ferulic acid is in the
range of 0.001-0.02 mol L-1. Ea was measured at 40-60 °C for all three systems.
In conclusion, this work examined lipase-catalyzed esterifica-
tion of ferulic acid with oleyl alcohol in an ionic liquid/isooctane
binary system. [Hmim][PF6]/isooctane and [Omim][PF6]/
isooctane systems are found to be capable of yielding high volu-
metric productivities of the ferulic acid oleyl ester. Substrate con-
centration, IL/isooctane volume ratio, and temperature are found
to be crucial parameters governing the volumetric productivity
and the reaction conditions of both systems, and have been opti-
mized individually. The optimum volumetric productivity of
48.12 mg/mL for [Hmim][PF6]/isooctane and 26.92 mg/mL
for [Omim][PF6]/ isooctane were obtained under their opti-
mized conditions.
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’ AUTHOR INFORMATION
Corresponding Author
*E-mail: chenbil@fjnu.edu.cn.
Funding Sources
This project was sponsored by the Department of Science and
Technology, Fujian Province (No. 2007I0039, 2008I0047), the
Natural Science Foundation, Fujian Province, China (No.
2011J01036), and the Scientific Research Foundation for the
Returned Overseas Chinese Scholars, State Education Ministry
of China.
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dx.doi.org/10.1021/jf104101z |J. Agric. Food Chem. 2011, 59, 1256–1263