Journal of Organic Chemistry p. 4608 - 4615 (1991)
Update date:2022-07-29
Topics:
Lyttle, Matthew H.
Wright, Peter B.
Sinha, Nanda D.
Bain, J. D.
Chamberlin, A. Richard
The 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diethylphosphoramidites) 3, 5, 7, and 9, modified monomers for RNA synthesis, were prepared from 2-cyanoethyl N,N-diethylchlorophosphoramidite (1).In conjunction with newly developed coupling protocols for automated solid-phase synthesis, they afforded synthetic oligoribonucleotides up to 74 base units in length.The performance of the new compounds was compared to the analogous 5'-O-(4,4'-dimethoxytrityl)-2'-O-(trialkylsilyl)ribonucleoside 3'-O-(2-cyanoethyl N,N-diisopropylphosphoramidites) 4, 6, 8, and 10.Complete removal of benzoyl groups from N2-benzoylguanosine, which was incorporated into some of the synthetic oligoribonucleotides, was demonstrated.Purification procedures by reverse phase HPLC and PAGE methods are also presented.
View MoreContact:86-536-8866010
Address:NO.4455 EAST FUSHOU ST. WEIFANG DEVELOPMENT ZONE, SHANDONG, CHINA
website:http://www.hybio.com.cn
Contact:+86 755 26588093
Address:No.9 Linkong West Street, Hengdian Street, Huangpi District, Wuhan City, China.
Beijing Wisdom Chemicals Co., Ltd.
Contact:+86-10-52350335
Address:F2, BLDG 19, Liando Valley U, Majuqiao, Tongzhou District, Beijing, China
Contact:+86-571-87010026
Address:202, Zhenhua Road,
jiangsu haian chemical co.,ltd.
Contact:86-513-15851283853
Address:No.99,Changjiang West Road,Haian County,Jiangsu Province,China
Doi:10.1007/s10593-011-0787-z
(2011)Doi:10.1134/S1070428011090284
(2011)Doi:10.1007/s10593-010-0487-0
(2010)Doi:10.1016/j.tetlet.2011.08.082
(2011)Doi:10.1021/ja01151a011
(1951)Doi:10.1021/ja00023a084
(1991)