T. Kálai et al. / Bioorg. Med. Chem. 19 (2011) 7311–7317
7317
0.05 M PBS to an absorbance of 0.70 ( 0.02) at 734 nm. The solu-
tion of antioxidant was added to ABTS+ solution in 12.5, 10, 7.5,
2.5 M concentration in ethanol or PBS. Trolox was also prepared
Supplementary data
l
Supplementary data associated with this article can be found, in
in ethanol or PBS as a standard. Absorbance reading was taken at
25 °C after 6 min incubation with antioxidant. Experiments were
performed on a Specord 40 spectrophotometer. The percentage
inhibition of absorbance at 734 nm is calculated and plotted as a
function of concentration of antioxidants and of Trolox. To calcu-
late the TEAC, the gradient of the plot of the percentage inhibition
of absorbance versus concentration plot for antioxidant in question
is divided by the gradient of the plot for Trolox. The TEAC values
and standard deviations are the results of three independent
experiments.
References and notes
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Acknowledgments
This work was supported by grants from the Hungarian Na-
tional Research Fund (OTKA–NKTH K67597, OTKA K 81123). The
authors thank to Viola Csokona for technical assistance and for ele-
mental analysis.
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