2258
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were determined for synthetic compounds against E. coli, S. aureus
and B. subtilis as bacteria and C. albicans and S. cerevisiae as yeasts.
It was carried out by impregnation of different concentrations of
was determined to the larvae of Artemia salina using brine shrimp
lethality bioassay. Different concentrations of each compound (10,
100 and 1000 lg/ml) were suspended in 5 mL vials containing sal-
synthesized compounds (0, 10, 50, 100, 1000
l
g/mL) in DMSO as
ine solution and 20 shrimps. Three replicates were used for each
concentration and living larvae were counted after 72 h. All data
were expressed as mean SD.41
a solvent and then placed on filter paper discs of the same diameter
(5 mm). The agar plate dilution method was used to inoculate the
bacteria and yeasts used in the plate. Nutrient agar medium was
seeded with 100
l
L of inoculum size (5 Â 105 for bacteria and
References and notes
4 Â 104 for yeasts). The impregnated discs containing the tested
samples of different concentrations were placed on the agar med-
ium seeded with tested microorganisms. Standard antibiotic discs
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isms. The antimicrobial activities of the tested samples were deter-
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l
After 72 h incubation, absorbance was measured at 546 nm. DMSO
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and 50
the above conditions. At the end of the incubation, 50
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for 4 h. The supernatant was decanted and DMSO (100 L) was
l
g/mL) were added and then incubated for 72 h under
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L of tetra-
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5.6. The lethal dose
Brine shrimps lethality bioassay is very simple bench-top assay
used to measure cytotoxicity of plant extracts as well as synthetic
compounds. Brine shrimp eggs, available commercially and being
used as fish food. Evaluation of natural products and synthetic
compounds by using brine shrimp describes not only cytotoxicity
but also anticancer, antiviral, insecticidal and pesticidal potential.
The cytotoxicity lethal dose (LD50) of the synthesized compounds
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