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Further injections were performed after 0.5, 1.75, 3.5 and 5 h of
incubation. Each experiment was done in duplicate. The
trifluoromenadione 3 and the lawsone 4 were analyzed by nega-
tive APCI-MS and detected by m/z peaks at 226 and 173 with tR
of 0.4 and 0.15 min, respectively. Known concentrations of
trifluoromenadione 3 and lawsone 4 solutions were used for the
calibration curves.
In vitro parasite cultures, antiparasitic and cytotoxicity bioassays
The CQ-sensitive 3D7 and the multi-resistant K1 and Dd2
strains of Plasmodium falciparum were cultivated using standard
protocols previously reported.5b,7
Determination of ED50 values against P. falciparum strains.
Drug susceptibility of P. falciparum was studied using a
modified method32 of the protocol described previously for the
3H-hypoxanthine incorporation-based assay.33 All assays
included CQ diphosphate (Sigma, UK) as standard and control
wells with untreated infected and uninfected erythrocytes. ED50
values were derived by sigmoidal regression analysis (Microsoft
xlfit™). All data from in vitro tests were carried out in triplicate
and were given with the 95% confidence limits
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(Alamar Blue) assay as previously described.7
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The authors are very thankful to Johannes Melcher, BZH and
Thomas Ruppert, ZMBH from Heidelberg University for
ESI-MS analysis of fluoro-reacted GR proteins. This work has
been funded in part by the collaborative center SFB544 (B14
project) and the International Center for Frontier Research in
lyses were performed at the TechMedILL Platform of the Unité
Mixte de Service CNRS-Université de Strasbourg (UMS 3286);
the authors are grateful to Patrick Gizzi for the data and the fruit-
ful discussions. The authors are indebted to Elena Cesar Rodo
for recording the IR spectra of the revised manuscript.
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