Fig. 3 Binding of 5-PA-InsP5 (2) to human PPIP5K2KD (a–c) and phosphorylation of 2 by PPIP5K2KD (d). (a) 5-PA-InsP5 (2, ball-and-stick
model) and its interacting residues (stick model) for hPPIP5K2 are shown in light blue for carbon, blue for nitrogen, orange and red for phosphate
groups. Residues that make contacts with 5-PP-InsP5 (green stick) are also shown in green carbon. Magnesium atoms are shown as spheres. C-4
and C-5 in the inositol ring are labelled. (b) Residues that interact with PA or PP are shown. Bond distances (dashed lines) are denoted in A.
(c) Key catalytic residues are shown in stick model. Inositol phosphates and nucleotide cofactors are shown in ball-and-stick model. Colours are as
(a). Also shown are catalytic magnesium atoms (magenta spheres). C-1 of the inositol ring is labelled. Bond distances (dashed lines) are denoted in A.
(d) Phosphorylation of 5-PA-InsP5 by PPIP5K2KD
.
Team (SER-CAT) 22-beamline at the Advanced Photon Source,
Argonne National Laboratory. Supporting institutions may be
Photon Source was supported by the U. S. Department of
Energy, Office of Science, Office of Basic Energy Sciences, under
Contract No. W-31-109-Eng-38.
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This journal is The Royal Society of Chemistry 2012