52
M. Napoli et al. / Journal of Organometallic Chemistry 725 (2013) 46e53
(NCHCH2CH2CH2CHOH
(NCHCH2CH2CH2CHOH
of
of
cyclopentyl
cyclopentyl
ring),
ring),
30.1
19.7
37 ꢁC, bacterial growth was checked by spectrophotometric reading
at a wavelength of 600 nm, a sample of bacterial cells grown in LB
medium without the addition of any compound was used as control.
For each experiment, carried out in duplicate, triplicate assays
were performed.
(NCHCH2CH2CH2CHOH of cyclopentyl ring).
Mass spectrum: 439 [Ag(L2)2]þ.
4.7. Synthesis of (3)
4.11. Cytotoxicity test by using brine shrimp assay
The complex was synthesized following the procedure
described for the complex 1, i.e. by reacting, in CH3CN (60 ml),
1.0 mmol of the ligand L3 (0.308 g) with 0.5 mmol of silver oxide
(0.116 g) at room temperature. The reaction mixture was then
refluxed for 12 h and then the solvent was concentrated at reduced
pressure and the product was obtained as white solid by precipi-
tation at ꢀ20 ꢁC and dried in vacuo.
The Brine shrimps (Artemia salina) assay was performed in
triplicate with appropriate amounts of samples dissolved in DMSO
(1% final volume) to reach final concentrations of 100, 10 and 1 mg/
ml, using 10 freshly hatched larvae suspended in 5 ml of artificial
sea water [22]. Briefly, for each dose tested, surviving shrimps were
left at RT and counted after 24 h, and the data statistically analyzed
by the Finney program [25], which affords LD50 values with 95%
confidence intervals.
Yield ¼ 18%.
Elemental analysis of (3): found (%): C 29.7, H 3.7, N 6.8, O 3.8, Ag
26.1, I 30.7. Calc. for C10H16AgIN2O (%): C 29.9, H 3.8, N 6.7, O 3.8, Ag
26.0; I 30.6.
1H NMR (DMSO-d6, 298 K): 7.53 (d, 1H, NCHCHN); 7.42 (d, 1H,
NCHCHN); 4.03 (m, 1H, CHOH); 3.86 (m, 1H, NCH); 3.83 (s, 3H,
NCH3); 2.30e1.50 (m, 8H, NCHCH2CH2CH2CH2CHOH of cyclohexyl
ring).
Acknowledgments
We wish to thank Italian Minister of University and Research for
financial support of this work. The authors are grateful to Dr. I.
Immediata, Dr. P. Oliva and Dr. P. Iannece for helpful technical
support.
13C {1H} NMR (DMSO-d6, 298 K): 178.9 (NCN); 122.5 (NCHCHN),
120.0 (NCHCHN), 71.3 (OCH2), 67.0 (NCH2), 39.5 (NCH3); 35.0
(NCHCH2CH2CH2CH2CHOH
(NCHCH2CH2CH2CH2CHOH
(NCHCH2CH2CH2CH2CHOH
of
of
of
cyclohexyl
cyclohexyl
cyclohexyl
ring),
ring),
ring),
33.0
24,8
23,9
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