790
KUMIRSKA et al.
heptose) but also resulted from the presence of the
digalactose side chain in the repeating unit. The opposite
trend was observed for the content of Glc, which is highꢀ
er in the highꢀmolecularꢀmass OPSI.
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Methylation analysis (Table 2) showed that the conꢀ
tent of terminal Glc decreased from the OPSI (1.79) to
OPSIII (1.13) corroborating the results of sugar analysis.
The same trend (a content reduction from 0.32 to 0.20)
was observed for 3,4ꢀdisubstituted GalNAc. This indicatꢀ
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the longer OPS chains and bear no galactose disaccharide.
This is the first structure established for the OPS
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This concurs with the results of Clark et al. [12], who
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Financial support was provided by the Polish
Ministry of Research and Higher Education (Grants
BW/8200ꢀ5ꢀ0475ꢀ0 and DS/8200ꢀ4ꢀ0085ꢀ11) and by the
Medical University of Gdansk (Grant No. W173).
BIOCHEMISTRY (Moscow) Vol. 76 No. 7 2011