Monoclonal ELISA for Chlorpyrifos
J. Agric. Food Chem., Vol. 44, No. 12, 1996 4061
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required.
Among the MAbs obtained one was selected for
further studies on the basis of its highest affinity to
chlorpyrifos. This MAb derived from the hapten that
better resembles the chlorpyrifos molecular structure,
since the spacer arm is an elongation of one of the
ethoxy groups. After the best enzyme-labeled hapten
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ABBREVIATIONS USED
BSA, bovine serum albumin; CR, cross-reactivity;
DCM, dichloromethane; DMF, N,N-dimethylformamide;
ELISA, enzyme-linked immunosorbent assay; EtAc,
ethyl acetate; HRP, horseradish peroxidase; Hx, hexane;
MAb, monoclonal antibody; I50, concentration giving
50% inhibition of maximum response; NHS, N-hydrox-
ysuccinimide; NMR, nuclear magnetic resonance; OPD,
o-phenylenediamine; OVA, ovalbumin; PBS, 10 mM
phosphate buffer, 137 mM NaCl, 2.7 mM KCl, pH 7.4;
PBST, PBS containing 0.05% Tween 20; PEG, poly-
(ethylene glycol); TCP, 3,5,6-trichloro-2-pyridinol; THF,
tetrahydrofuran; TLC, thin-layer chromatography.
ACKNOWLEDGMENT
We thank M. Adelantado and R. Nello for technical
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