Tetrahedron p. 6373 - 6386 (1988)
Update date:2022-07-29
Topics:
Pradines, A.
Klaebe, A.
Perie, J.
Paul, F.
Monsan, P.
Title compounds were synthesized on a preparative scale using alkaline phosphatase, orthophosphoric monoester phosphohydrolase E.C. 3.1.3.1., in reverse hydrolysis conditions.Optimization for one of the 25 phosphoryl acceptors investigated (glycerol) shows that up to 55percent synthesis yield can be obtained using a large excess of substrate, conditions in which the enzymatic activity remains high.From the results obtained with different phosphoryl group donors, phosphate, pyrophosphate and polyphosphates and with enzymes of different sources, it comes up that the best results are obtained with pyrophosphate and with the weakly purified calf intestine alkaline phosphatase.The extent of enzymatic hydrolysis of the donor can be reduced owing to the existence of two different pH optima for the two reactions, phosphorylation and hydrolysis.The synthesis can be also performed using inert co-solvents which allow to reduce the amount of acceptor used, as long as Zn++ is added to the reaction medium.The results are discussed in terms of the catalytic mechanism of alkaline phosphatase.
View Morexi'an taima biological engineering co., ltd.
Contact:+8615619038117
Address:6 No,keji road xi'an city of china
Zhejiang Golden-Shell Pharmaceutical Co.,Ltd.
Contact:+86-576-87501888 / 87501988
Address:No.89 Zhongxing Road. Li'ao, Yuhuan, Zhejiang, China
Shanghai Sunway Pharmaceutical Technology Co.,Ltd.
Contact:+86-21-5161 3915
Address:Shanghai YangPu
Suzhou Credit International Trading Co., Ltd
Contact:+86-512-65398039
Address:Qingdeng, Hightech. District, Suzhou
Contact:13357117572
Address:No.149 Shiji dadao Road.
Doi:10.1021/ja01262a003
(1942)Doi:10.1016/S0040-4039(00)87101-4
(1982)Doi:10.1021/jo01033a011
(1964)Doi:10.1246/bcsj.60.981
(1987)Doi:10.1016/S0040-4039(96)02018-7
(1996)Doi:10.1021/ja993401r
(2000)